Novel Alcaligenes ammonioxydans sp. nov. from wastewater treatment sludge oxidizes ammonia to N2 with a previously unknown pathway

被引:60
作者
Wu, Meng-Ru [1 ,2 ,3 ]
Hou, Ting-Ting [1 ,2 ,3 ]
Liu, Ying [1 ,2 ]
Miao, Li-Li [1 ,2 ]
Ai, Guo-Min [1 ,2 ]
Ma, Lan [1 ,2 ,3 ]
Zhu, Hai-Zhen [1 ,2 ,3 ]
Zhu, Ya-Xin [1 ,2 ]
Gao, Xi-Yan [1 ,2 ,3 ]
Herbold, Craig W. [4 ]
Wagner, Michael [4 ,5 ]
Li, De-Feng [1 ,2 ,3 ]
Liu, Zhi-Pei [1 ,2 ,3 ]
Liu, Shuang-Jiang [1 ,2 ,3 ,6 ]
机构
[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China
[2] Chinese Acad Sci, Inst Microbiol, Environm Microbiol Res Ctr, Beijing, Peoples R China
[3] Univ Chinese Acad Sci, Beijing, Peoples R China
[4] Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Vienna, Austria
[5] Aalborg Univ, Ctr Microbial Communities, Aalborg, Denmark
[6] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266273, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
HETEROTROPHIC NITRIFICATION; THIOSPHAERA-PANTOTROPHA; AEROBIC DENITRIFICATION; PYRUVIC-OXIME; HYDROXYLAMINE OXIDASE; PURIFICATION; FAECALIS; NITROGEN; OXIDATION; REDUCTASE;
D O I
10.1111/1462-2920.15751
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Heterotrophic nitrifiers are able to oxidize and remove ammonia from nitrogen-rich wastewaters but the genetic elements of heterotrophic ammonia oxidation are poorly understood. Here, we isolated and identified a novel heterotrophic nitrifier, Alcaligenes ammonioxydans sp. nov. strain HO-1, oxidizing ammonia to hydroxylamine and ending in the production of N-2 gas. Genome analysis revealed that strain HO-1 encoded a complete denitrification pathway but lacks any genes coding for homologous to known ammonia monooxygenases or hydroxylamine oxidoreductases. Our results demonstrated strain HO-1 denitrified nitrite (not nitrate) to N-2 and N2O at anaerobic and aerobic conditions respectively. Further experiments demonstrated that inhibition of aerobic denitrification did not stop ammonia oxidation and N-2 production. A gene cluster (dnfT1RT2ABCD) was cloned from strain HO-1 and enabled E. coli accumulated hydroxylamine. Sub-cloning showed that genetic cluster dnfAB or dnfABC already enabled E. coli cells to produce hydroxylamine and further to N-15(2) from ((NH4)-N-15)(2)SO4. Transcriptome analysis revealed these three genes dnfA, dnfB and dnfC were significantly upregulated in response to ammonia stimulation. Taken together, we concluded that strain HO-1 has a novel dnf genetic cluster for ammonia oxidation and this dnf genetic cluster encoded a previously unknown pathway of direct ammonia oxidation (Dirammox) to N-2.
引用
收藏
页码:6965 / 6980
页数:16
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