Novel Alcaligenes ammonioxydans sp. nov. from wastewater treatment sludge oxidizes ammonia to N2 with a previously unknown pathway

被引：61

作者：

Wu, Meng-Ru ^{[1
,2
,3
]}

Hou, Ting-Ting ^{[1
,2
,3
]}

Liu, Ying ^{[1
,2
]}

Miao, Li-Li ^{[1
,2
]}

Ai, Guo-Min ^{[1
,2
]}

Ma, Lan ^{[1
,2
,3
]}

Zhu, Hai-Zhen ^{[1
,2
,3
]}

Zhu, Ya-Xin ^{[1
,2
]}

Gao, Xi-Yan ^{[1
,2
,3
]}

Herbold, Craig W. ^{[4
]}

Wagner, Michael ^{[4
,5
]}

Li, De-Feng ^{[1
,2
,3
]}

Liu, Zhi-Pei ^{[1
,2
,3
]}

Liu, Shuang-Jiang ^{[1
,2
,3
,6
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, Environm Microbiol Res Ctr, Beijing, Peoples R China

[3] Univ Chinese Acad Sci, Beijing, Peoples R China

[4] Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Microbial Ecol, Vienna, Austria

[5] Aalborg Univ, Ctr Microbial Communities, Aalborg, Denmark

[6] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266273, Peoples R China

来源：

ENVIRONMENTAL MICROBIOLOGY | 2021年 / 23卷 / 11期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

HETEROTROPHIC NITRIFICATION; THIOSPHAERA-PANTOTROPHA; AEROBIC DENITRIFICATION; PYRUVIC-OXIME; HYDROXYLAMINE OXIDASE; PURIFICATION; FAECALIS; NITROGEN; OXIDATION; REDUCTASE;

D O I：

10.1111/1462-2920.15751

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Heterotrophic nitrifiers are able to oxidize and remove ammonia from nitrogen-rich wastewaters but the genetic elements of heterotrophic ammonia oxidation are poorly understood. Here, we isolated and identified a novel heterotrophic nitrifier, Alcaligenes ammonioxydans sp. nov. strain HO-1, oxidizing ammonia to hydroxylamine and ending in the production of N-2 gas. Genome analysis revealed that strain HO-1 encoded a complete denitrification pathway but lacks any genes coding for homologous to known ammonia monooxygenases or hydroxylamine oxidoreductases. Our results demonstrated strain HO-1 denitrified nitrite (not nitrate) to N-2 and N2O at anaerobic and aerobic conditions respectively. Further experiments demonstrated that inhibition of aerobic denitrification did not stop ammonia oxidation and N-2 production. A gene cluster (dnfT1RT2ABCD) was cloned from strain HO-1 and enabled E. coli accumulated hydroxylamine. Sub-cloning showed that genetic cluster dnfAB or dnfABC already enabled E. coli cells to produce hydroxylamine and further to N-15(2) from ((NH4)-N-15)(2)SO4. Transcriptome analysis revealed these three genes dnfA, dnfB and dnfC were significantly upregulated in response to ammonia stimulation. Taken together, we concluded that strain HO-1 has a novel dnf genetic cluster for ammonia oxidation and this dnf genetic cluster encoded a previously unknown pathway of direct ammonia oxidation (Dirammox) to N-2.

引用

页码：6965 / 6980

页数：16

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