Effect of substrate feeding on production of fructosyltransferase by Penicillium purpurogenum

被引:27
作者
Dhake, A. B. [1 ]
Patil, M. B. [1 ]
机构
[1] Nagpur Univ, Univ Dept Biochem, LIT Premises, Nagpur 440033, Maharashtra, India
关键词
Fructooligosaccharides; frutosyltransferase; Penicillium purpurogenum;
D O I
10.1590/S1517-83822007000200002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Penicillium purpurogenum was found to produce both extracellular and intracellular fructosyltransferase. The organism could also produce sucrose hydrolytic enzyme. Sucrose was found to be the best carbon source for fructosyltransferase production. Maximum intracellular and extracellular fructosyltransferase production was observed after 3(rd) and 4(th) day of cultivation, respectively. The enzyme activity was optimum at temperature of 55 degrees C and pH 5.5. The addition of amino acids, like leucine induced slightly the extracellular fructosyltransferase production, where as histidine and leucine had little inductive effect on intracellular fructosyltransferase production. Enhanced production of fructosyltransferase by Penicillium purpurogenum was observed when sucrose content was restored by additional sucrose feeding to the cultivation medium during production period.
引用
收藏
页码:194 / 199
页数:6
相关论文
共 33 条
[1]   Effect of amino acids on production of xylanase and pectinase from Streptomyces sp QG-11-3 [J].
Beg, QK ;
Bhushan, B ;
Kapoor, M ;
Hoondal, GS .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2000, 16 (02) :211-213
[2]   Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats [J].
Campbell, JM ;
Fahey, GC ;
Wolf, BW .
JOURNAL OF NUTRITION, 1997, 127 (01) :130-136
[3]   Production of beta-fructofuranosidase by Aspergillus japonicus [J].
Chen, WC ;
Liu, CH .
ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (02) :153-160
[4]   Production, properties and applications of food-grade oligosaccharides [J].
Crittenden, RG ;
Playne, MJ .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1996, 7 (11) :353-361
[5]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[6]   Production of fructo-oligosaccharides by levansucrase from Bacillus subtilis C4 [J].
Euzenat, O ;
Guibert, A ;
Combes, D .
PROCESS BIOCHEMISTRY, 1997, 32 (03) :237-243
[7]  
FISHBEIN L, 1988, VET HUM TOXICOL, V30, P104
[8]  
Griffin DH, 1994, FUNGAL PHYSL, V2, P458
[9]   Enhanced production of xylanase from Staphylococcus sp SG-13 using amino acids [J].
Gupta, S ;
Bhushan, B ;
Hoondal, GS .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1999, 15 (04) :511-512
[10]   PURIFICATION AND PROPERTIES OF A FRUCTOOLIGOSACCHARIDE-PRODUCING BETA-FRUCTOFURANOSIDASE FROM ASPERGILLUS-NIGER ATCC-20611 [J].
HIRAYAMA, M ;
SUMI, N ;
HIDAKA, H .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (03) :667-673