Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method

被引：21
作者：
Chen, Ming [1 ]
Lin, Yong-Qing [1 ]
Xie, Shuang-Lun [1 ]
Wu, Hong-Fu [2 ]
Wang, Jing-Feng [1 ]
机构：
[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Cardiol, Guangzhou 510275, Guangdong, Peoples R China
[2] Guangdong Med Coll, Dept Physiol, Dongguan, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 04期
基金：
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词：
Cardiomyocytes; Differentiation; Embryonic stem cells; Hanging drop method; CARDIOMYOCYTE DIFFERENTIATION; IN-VITRO;
D O I：
10.1007/s10529-010-0494-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Hanging drop (HD) culture is used to induce differentiation of embryonic stem cells (ESCs) into other cell types including cardiomyocytes. However, the factors affecting cardiac differentiation of ESCs with this method remain incompletely understood. We have investigated the effects of the starting number of ESCs in embryoid bodies (EBs) and the time of EB adherence to gelatin-coated plates on cardiac differentiation: cardiac differentiation was increased in the EBs by a larger number of ESCs and was decreased by plating EBs at day 4 or earlier. These two factors can thus be optimized to enrich the cardiac differentiation in ESCs using the HD method.
引用
收藏
页码：853 / 858
页数：6
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