Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis

被引：52

作者：

Conley, Andrew J. ^{[1
,2
]}

Zhu, Hong ^{[1
]}

Le, Linda C. ^{[1
]}

Jevnikar, Anthony M. ^{[3
]}

Lee, Byong H. ^{[4
]}

Brandle, Jim E. ^{[1
,5
]}

Menassa, Rima ^{[1
]}

机构：

[1] Agr & Agri Food Canada, So Crop Protect & Food Res Ctr, London, ON, Canada

[2] Univ Western Ontario, Dept Biol, London, ON, Canada

[3] Lawson Hlth Res Inst, Transplantat Immunol Grp, London, ON, Canada

[4] Agr & Agri Food Canada, Food Res & Dev Ctr, St Hyacinthe, PQ, Canada

[5] Vineland Res & Innovat Ctr, Vineland Stn, ON, Canada

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2011年 / 9卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

molecular farming; recombinant protein production; transgenic plants; transient expression; erythropoietin; interleukin-10; alpha-amylase; Pseudomonas aeruginosa; EXTRACELLULAR ALPHA-AMYLASE; B SURFACE-ANTIGEN; TRANSIENT EXPRESSION; GENE-EXPRESSION; HIGH-LEVEL; ENDOPLASMIC-RETICULUM; POLYPEPTIDE FUSIONS; PYROCOCCUS-FURIOSUS; PLANTS; ANTIBODIES;

D O I：

10.1111/j.1467-7652.2010.00563.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

P>Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin-10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable alpha-amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.

引用

页码：434 / 444

页数：11

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