Electrochemical study of the interaction between cytochrome c and DNA at a modified gold electrode

被引：17

作者：

Ding, XQ ^{[1
]}

Li, JH ^{[1
]}

Hu, JB ^{[1
]}

Li, QL ^{[1
]}

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 339卷 / 01期

基金：

中国国家自然科学基金;

关键词：

cytochrome c; DNA; electron-transfer kinetics; gold electrode; EQCM; AFM;

D O I：

10.1016/j.ab.2004.11.021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The direct electron transfer of surface-confined horse heart cytochrome c (Cyt c) was achieved using COOH-terminated alkanethiolate-modified gold electrode. Later DNA was immobilized on the two-layer modified electrode. The quantitative determination of DNA was explored and the interaction between cytochrome c and DNA was studied. The binding site sizes were determined to be 15 bp per Cyt c molecule with double-stranded (ds) DNA and 30 nucleotides binding one Cyt c molecule with single-stranded (ss) DNA. At the dsDNA/Cyt c/MUA/Au electrode, the rate constant of oxidation electron transfer k(s,ox) = 1.59 x 10(-3) cm s(-1) was obtained, at the ssDNA/Cyt c/MUA/Au electrode, the value was 2.43 x 10(-3) m s(-1) when the scan rate was 1.0 V/s. The different electrodes were characterized with electrochemical quartz crystal microbalance and atomic force microscope. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：46 / 53

页数：8

共 32 条

[1] The transient nature of the diffusion controlled component of the electrochemistry of cytochrome c at 'bare' gold electrodes: an explanation based on a self-blocking mechanism [J].

Allen, H ;

Hill, O ;

Hunt, NI ;

Bond, AM .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1997, 436 (1-2) :17-25

[2] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS [J].

ARMSTRONG, FA ;

HILL, HAO ;

WALTON, NJ .

ACCOUNTS OF CHEMICAL RESEARCH, 1988, 21 (11) :407-413

[3]

ARMSTRONG FA, 1984, J ELECTROANAL CHEM, V178, P69

[4] INTERFACIAL ELECTROCHEMISTRY OF CYTOCHROME-C AT TIN OXIDE, INDIUM OXIDE, GOLD, AND PLATINUM-ELECTRODES [J].

BOWDEN, EF ;

HAWKRIDGE, FM ;

BLOUNT, HN .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1984, 161 (02) :355-376

[5] THE DIRECT ELECTROCHEMISTRY OF CYTOCHROME-C AT A GOLD MICROBAND ELECTRODE MODIFIED WITH 4,6-DIMETHYL-2-MERCAPTOPYRIMIDINE [J].

CAI, CX .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1995, 393 (1-2) :119-122

[6] The bioelectrocatalytic properties of cytochrome C by direct electrochemistry on DNA film modified electrode [J].

Chen, SM ;

Chen, SV .

ELECTROCHIMICA ACTA, 2003, 48 (05) :513-529

[7] Characterization of the direct electron transfer and bioelectrocatalysis of horseradish peroxidase in DNA film at pyrolytic graphite electrode [J].

Chen, XH ;

Ruan, CM ;

Kong, JL ;

Deng, JQ .

ANALYTICA CHIMICA ACTA, 2000, 412 (1-2) :89-98

[8] Hemin-stimulated docking of cytochrome c to a hemin-DNA aptamer complex [J].

Chinnapen, DJF ;

Sen, D .

BIOCHEMISTRY, 2002, 41 (16) :5202-5212

[9] VOLTAMMETRY OF COVALENTLY IMMOBILIZED CYTOCHROME-C ON SELF-ASSEMBLED MONOLAYER ELECTRODES [J].

COLLINSON, M ;

BOWDEN, EF ;

TARLOV, MJ .

LANGMUIR, 1992, 8 (05) :1247-1250

[10] Self-assembled monolayers of thiols on gold electrodes for bioelectrochemistry and biosensors [J].

Dong, SJ ;

Li, JH .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1997, 42 (01) :7-13

← 1 2 3 4 →