Dose response chemopreventive potential of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats

被引：36

作者：

Rajakumar, Thangarasu ^{[1
]}

Pugalendhi, Pachaiappan ^{[1
]}

Thilagavathi, Subbaiyan ^{[1
]}

机构：

[1] Annamalai Univ, Fac Sci, Dept Biochem & Biotechnol, Annamalainagar 608002, Tamil Nadu, India

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2015年 / 231卷

关键词：

Mammary carcinogenesis; 7,12-Dimethylbenz(a)anthracene; Allyl isothiocyanate; Biotransformation enzymes; LOW-DENSITY-LIPOPROTEIN; BREAST-CANCER; OXIDATIVE STRESS; CRUCIFEROUS VEGETABLES; BENZYL ISOTHIOCYANATE; LIPID PEROXIDES; ANIMAL-TISSUES; G(2)/M ARREST; IN-VIVO; ENZYMES;

D O I：

10.1016/j.cbi.2015.02.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study aimed to investigate the dose response chemopreventive potential of allyl isothiocyanate (AITC) against 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague-Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. We observed reduced body weight and increased in total number of tumors, tumor incidence and tumor volume in DMBA-induced rats. We also observed decreased antioxidant status (SOD, CAT, GPX and GSH) and increased lipid peroxidation (TBARS and LOOH) in plasma and mammary tissues. Increased levels of CYP450, Cyt-b5 and decreased levels of phase II (GST and GR) biotransformation enzymes noticed in liver and mammary tissues of DMBA-induced rats. Further, increased levels of lipid profile (TC, TG, PL and FFA) and lipoprotein (LDL and VLDL) were noticed. Whereas, decreased level of HDL in plasma and decreased levels of PL and FFA in mammary tissue. Oral administration of AITC different doses (10, 20 and 40 mg/kg bw) inhibited the tumor incidence and restored levels of biochemical markers. Biochemical findings are supported by histopathological studies. These results suggested that AITC at a dose of 20 mg/kg bw significantly exert chemopreventive potential against DMBA-induced mammary carcinogenesis. (C) 2015 Published by Elsevier Ireland Ltd.

引用

页码：35 / 43

页数：9

共 73 条

[1] The level of antioxidant enzymes, plasma vitamins C and E in cement plant workers [J].

Aydin, S ;

Aral, I ;

Kilic, N ;

Bakan, I ;

Aydin, S ;

Erman, F .

CLINICA CHIMICA ACTA, 2004, 341 (1-2) :193-198

[2] PLASMA-LIPIDS AND PROLACTIN IN PATIENTS WITH BREAST-CANCER [J].

BANI, IA ;

WILLIAMS, CM ;

BOULTER, PS ;

DICKERSON, JWT .

BRITISH JOURNAL OF CANCER, 1986, 54 (03) :439-446

[3] EFFECT OF SODIUM NITRITE ON RED CELL GSH [J].

BEUTLER, E ;

KELLY, BM .

EXPERIENTIA, 1963, 19 (02) :96-&

[4] The principal urinary metabolite of allyl isothiocyanate, N-acetyl-S-(N-allylthiocarbamoyl)cysteine, inhibits the growth and muscle invasion of bladder cancer [J].

Bhattacharya, Arup ;

Li, Yun ;

Geng, Feng ;

Munday, Rex ;

Zhang, Yuesheng .

CARCINOGENESIS, 2012, 33 (02) :394-398

[5]

BURSTEIN M, 1970, J LIPID RES, V11, P583

[6]

CARLBERG I, 1985, METHOD ENZYMOL, V113, P484

[7] HIGH-DENSITY-LIPOPROTEIN IS A SCAVENGER OF SUPEROXIDE ANIONS [J].

CHANDER, R ;

KAPOOR, NK .

BIOCHEMICAL PHARMACOLOGY, 1990, 40 (07) :1663-1665

[8] Allyl isothiocyanate triggers G2/M phase arrest and apoptosis in human brain malignant glioma GBM 8401 cells through a mitochondria-dependent pathway [J].

Chen, Nian-Gu ;

Chen, Kuan-Tin ;

Lu, Chi-Cheng ;

Lan, Yu-Hsuan ;

Lai, Cheng-Hung ;

Chung, Yang-Tsung ;

Yang, Jai-Sing ;

Lin, Yung-Chang .

ONCOLOGY REPORTS, 2010, 24 (02) :449-455

[9] Influence of selenium on glutathione and some associated enzymes in rats with mammary tumor induced by 7,12-dimethylbenz(a)anthracene [J].

Chidambaram, N ;

Baradarajan, A .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 1996, 156 (02) :101-107

[10] Effect of Withania somnifera on DMBA induced carcinogenesis [J].

Davis, L ;

Kuttan, G .

JOURNAL OF ETHNOPHARMACOLOGY, 2001, 75 (2-3) :165-168

← 1 2 3 4 5 6 7 8 →