Cryopreservation and in vitro culture of caprine preantral follicles

被引:32
|
作者
Figueiredo, J. R. [1 ]
Rodrigues, A. P. R. [1 ]
Silva, J. R. V. [2 ]
Santos, R. R. [3 ,4 ]
机构
[1] Univ Estadual Ceara, Fac Vet, Lab Manipulat Oocytes & Preantral Follicles LAMOF, Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Biotechnol Nucleus Sobral NUBIS, Sobral, Ceara, Brazil
[3] Univ Utrecht, Fac Vet Med, Vet Pharmaceut Pharmacol & Toxicol Div, Dept Equine Sci, NL-6584 CM Utrecht, Netherlands
[4] Fed Univ Para, Lab Biol & Med Wild Mammals Amazonia, BR-66075110 Belem, Para, Brazil
关键词
embryo; freezing; gene expression; goat; IVC; vitrification; SOLID-SURFACE VITRIFICATION; FIBROBLAST GROWTH FACTOR-2; GOAT PRIMORDIAL FOLLICLES; HUMAN OVARIAN TISSUE; STIMULATING-HORMONE; FOLLICULAR DEVELOPMENT; DIFFERENT TEMPERATURES; PROTEIN LOCALIZATION; SECONDARY FOLLICLES; INCUBATION TIMES;
D O I
10.1071/RD10227
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Preantral follicles (PFs) form a far larger oocyte reservoir (similar to 90% of the follicular population) than antral follicles. Several laboratories have focussed efforts on cryopreservation and in vitro culture (IVC) of PFs to obtain large numbers of fertilisable oocytes. This technology could be used to improve the reproductive potential of economically important animals, including goats, to preserve endangered species and breeds and improve fertility after chemotherapy in young women. Caprine PFs have been successfully cryopreserved using either vitrification or slow freezing. In addition, in vitro embryo production from oocytes enclosed in caprine PFs grown and matured in vitro was also achieved. The present paper selectively reviews the published studies on cryopreservation and IVC of caprine PFs to highlight advances, limitations and prospects.
引用
收藏
页码:40 / 47
页数:8
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