Active circulation of bluetongue vaccine virus serotype-2 among unvaccinated cattle in central Italy

被引：66

作者：

Ferrari, G ^{[1
]}

De Liberato, C ^{[1
]}

Scavia, G ^{[1
]}

Lorenzetti, R ^{[1
]}

Zini, M ^{[1
]}

Farina, F ^{[1
]}

Magliano, A ^{[1
]}

Cardeti, G ^{[1
]}

Scholl, F ^{[1
]}

Guidoni, M ^{[1
]}

Scicluna, MT ^{[1
]}

Amaddeo, D ^{[1
]}

Scaramozzino, P ^{[1
]}

Autorino, GL ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Reg Lazio & Toscana, I-00178 Rome, Italy

来源：

PREVENTIVE VETERINARY MEDICINE | 2005年 / 68卷 / 2-4期

关键词：

bluetongue virus; cattle-microbiological disease; vaccine; vaccine virus transmission; Culicoides; Italy;

D O I：

10.1016/j.prevetmed.2004.11.011

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Several seroconversions occurring in 2002 among sentinel cattle during the bluetongue-vaccination campaign in Lazio and Tuscany (central Italy) led to the suspicion of vaccine-virus circulation. Therefore in 2003, 17 seroconverting sentinel herds were investigated for the characteristics of the virus involved. From these farms, 91 unvaccinated animals and 57 Culicoides pools were tested for the presence of the bluetongue vaccine virus (serotype-2) or other strains. The presence of vaccine virus serotype-2 was confirmed by PCR followed by restriction analysis in the whole blood of 17 unvaccinated sentinel cattle and 12 pools of Culicoides imicola or C. obsoletus. Of the 17 herds, five were positive only for vaccine virus serotype-2, four were positive for other strains and two for both the vaccine and other strains; the remaining premises were virologicaly negative. The vaccine virus serotype-2 also was detected in areas not included in the vaccination campaign. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：103 / 113

页数：11

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