Enzyme immobilization onto renewable polymeric matrixes: Past, present, and future trends

被引:99
作者
Bezerra, Camilla Salviano [1 ]
Gentil de Farias Lemos, Celina Maria [1 ]
de Sousa, Marylane [1 ]
Barros Goncalves, Luciana Rocha [1 ]
机构
[1] Univ Fed Ceara, Dept Engn Quim, BR-60440554 Fortaleza, Ceara, Brazil
关键词
biomaterials; biopolymers and renewable polymers; catalysts; MULTIPOINT COVALENT ATTACHMENT; BOVINE SERUM-ALBUMIN; BETA-GALACTOSIDASE; ALPHA-AMYLASE; SILK FIBROIN; PROTEIN IMMOBILIZATION; SURFACE IMMOBILIZATION; ALCOHOL-DEHYDROGENASE; ACTIVATED SUPPORTS; CANDIDA-ANTARCTICA;
D O I
10.1002/app.42125
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
In this review, we present an overview of the different renewable polymers that are currently being used as matrixes for enzyme immobilization and their properties and of new developments in biocatalysts preparation and applications. Polymers obtained from renewable resources have attracted much attention in recent years because they are environmentally friendly and available in large quantities from natural sources. Different methods for the immobilization of enzymes with these matrixes are reviewed, in particular: (1) binding to a prefabricated biopolymer, (2) entrapment, and (3) crosslinking of enzyme molecules. Emphasis is given to relatively recent developments, such as the use of novel supports, novel entrapment methods and protocols of polymer derivatization, and the crosslinking of enzymes. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42125.
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页数:15
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