A caged nicotine with nanosecond range kinetics and visible light sensitivity

被引：53

作者：

Filevich, Oscar ^{[1
]}

Salierno, Marcelo ^{[1
]}

Etchenique, Roberto ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis, INQUIMAE, Buenos Aires, DF, Argentina

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2010年 / 104卷 / 12期

关键词：

Caged compound; Ruthenium complex; Nicotine; Leech; Neurotransmitter; nAChR; GLUTAMATE RECEPTORS; 2-PHOTON; PHOTOCHEMISTRY; ADDICTION; COMPLEXES; RUTHENIUM; LEECH; CELLS;

D O I：

10.1016/j.jinorgbio.2010.08.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the synthesis, characterization and applications of a ruthenium-bipyridine based caged nicotine. The complex [Ru(bpy)(2)(nic)(2)](2+) (where bpy = 2,2' bipyridine and nic = nicotine (3-[(2S)-1-methylpyrrolidin-2-yl] pyridine)) releases nicotine with a quantum yield phi = 0.23 upon irradiation with biologically harmless, blue (473 nm) or green (532 nm) light. The photolysis reaction is clean and very fast, with a time constant of 17 ns. The synthesis is simple and the obtained compound is characterized by NMR, UV-Vis spectroscopy and cyclic voltametry. We find that this compound is active in biological systems, being able to elicit action potentials in leech neurons. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：1248 / 1251

页数：4

共 23 条

[1] Bard A.J, 2002, Student Solutions Manual to accompany Electrochemical Methods: Fundamentals and Applicaitons, V2e
[2] Photochemistry and photophysics of coordination compounds: Ruthenium
Campagna, Sebastiano
Puntoriero, Fausto
Nastasi, Francesco
Bergamini, Giacomo
Balzani, Vincenzo
[J]. PHOTOCHEMISTRY AND PHOTOPHYSICS OF COORDINATION COMPOUNDS I, 2007, 280 : 117 - 214
[3] Nicotine addiction and nicotinic receptors: lessons from genetically modified mice
Changeux, Jean-Pierre
[J]. NATURE REVIEWS NEUROSCIENCE, 2010, 11 (06) : 389 - 401
[4] Molecular and cellular aspects of nicotine abuse
Dani, JA
Heinemann, S
[J]. NEURON, 1996, 16 (05) : 905 - 908
[5] PHOTOCHEMISTRY OF RU(BPY)32+
DURHAM, B
CASPAR, JV
NAGLE, JK
MEYER, TJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (18) : 4803 - 4810
[6] CIS-TRANS PHOTOISOMERIZATION IN RU(BPY)2(OH2)22+ - CRYSTAL-STRUCTURE OF TRANS-[RU(BPY)2(OH2)(OH)](CLO4)2
DURHAM, B
WILSON, SR
HODGSON, DJ
MEYER, TJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (02) : 600 - 607
[7] Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells
Fedoryak, OD
Sul, JY
Haydon, PG
Ellis-Davies, GCR
[J]. CHEMICAL COMMUNICATIONS, 2005, (29) : 3664 - 3666
[8] Genetics of dopamine receptors and drug addiction: a comprehensive review
Le Foll, Bernard
Gallo, Alexandra
Le Strat, Yann
Lu, Lin
Gorwood, Philip
[J]. BEHAVIOURAL PHARMACOLOGY, 2009, 20 (01): : 1 - 17
[9] ELECTROCHEMICAL PARAMETRIZATION OF METAL-COMPLEX REDOX POTENTIALS, USING THE RUTHENIUM(III) RUTHENIUM(II) COUPLE TO GENERATE A LIGAND ELECTROCHEMICAL SERIES
LEVER, ABP
[J]. INORGANIC CHEMISTRY, 1990, 29 (06) : 1271 - 1285
[10] Flash photolysis reveals a diversity of ionotropic glutamate receptors on the mitral cell somatodendritic membrane
Lowe, G
[J]. JOURNAL OF NEUROPHYSIOLOGY, 2003, 90 (03) : 1737 - 1746

← 1 2 3 →