WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes

被引：198

作者：

Davies, RC

Calvio, C

Bratt, E

Larsson, SH

Lamond, AI

Hastie, ND ^{[1
]}

机构：

[1] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland

[2] European Mol Biol Lab, Gene Express Programme, D-69012 Heidelberg, Germany

[3] Karolinska Inst, CMB, S-17177 Stockholm, Sweden

[4] Univ Dundee, Dept Biochem, Dundee DD1 4HN, Scotland

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 20期

基金：

英国惠康基金;

关键词：

WT1; U2AF65; spliceosome; alternative splicing;

D O I：

10.1101/gad.12.20.3217

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

WT1 is essential for normal kidney development, and genetic alterations are associated with Wilms' tumor, Denys Drash (DDS), and Frasier syndromes. Although generally considered a transcription factor this study has revealed that WT1 interacts with an essential splicing factor, U2AF65, and associates with the splicing machinery. WT1 is alternatively spliced and isoforms that include three amino acids, KTS, show stronger interaction with U2AF65 in vitro and better colocalization with splicing factors in vivo. Interestingly a mutation associated with DDS enhanced both -KTS WT1 binding to U2AE65 and splicing-factor colocalization. These data illustrate the functional importance of WT1 isoforms and suggest that WT1 plays a role in pre-mRNA splicing.

引用

页码：3217 / 3225

页数：9

共 42 条

[1] Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals [J].