1000 spider silkomes: Linking sequences to silk physical properties

被引：87

作者：

Arakawa, Kazuharu ^{[1
,2
,3
,4
]}

Kono, Nobuaki ^{[1
,3
]}

Malay, Ali D. ^{[5
]}

Tateishi, Ayaka ^{[5
,6
]}

Ifuku, Nao ^{[5
]}

Masunaga, Hiroyasu ^{[7
]}

Sato, Ryota ^{[5
,8
]}

Tsuchiya, Kousuke ^{[5
,6
]}

Ohtoshi, Rintaro ^{[5
,8
]}

Pedrazzoli, Daniel ^{[8
]}

Shinohara, Asaka ^{[8
]}

Ito, Yusuke ^{[8
]}

Nakamura, Hiroyuki ^{[5
,8
]}

Tanikawa, Akio ^{[9
]}

Suzuki, Yuya ^{[10
,11
]}

Ichikawa, Takeaki ^{[12
]}

Fujita, Shohei ^{[13
]}

Fujiwara, Masayuki ^{[1
]}

Tomita, Masaru ^{[1
,2
,3
]}

Blamires, Sean J. ^{[14
,27
]}

Chuah, Jo-Ann ^{[5
]}

Craig, Hamish ^{[5
,14
]}

Foong, Choon P. ^{[5
,6
]}

Greco, Gabriele ^{[15
]}

Guan, Juan ^{[16
]}

Holland, Chris ^{[17
]}

Kaplan, David L. ^{[18
]}

Sudesh, Kumar ^{[19
]}

Mandal, Biman B. ^{[20
,21
,22
]}

Norma-Rashid, Y. ^{[23
]}

Oktaviani, Nur A. ^{[5
]}

Preda, Rucsanda C. ^{[18
]}

Pugno, Nicola M. ^{[15
,24
]}

Rajkhowa, Rangam ^{[25
]}

Wang, Xiaoqin ^{[26
]}

Yazawa, Kenjiro ^{[5
]}

Zheng, Zhaozhu ^{[26
]}

Numata, Keiji ^{[5
,6
]}

机构：

[1] Keio Univ, Inst Adv Biosci, Tsuruoka, Yamagata 9970017, Japan

[2] Keio Univ, Fac Environm & Informat Studies, Fujisawa, Kanagawa 2528520, Japan

[3] Keio Univ, Grad Sch Media & Governance, Fujisawa, Kanagawa 2528520, Japan

[4] Exploratory Res Ctr Life & Living Syst ExCELLS, Natl Inst Nat Sci, Okazaki, Aichi 4448787, Japan

[5] RIKEN Ctr Sustainable Resource Sci, Biomacromol Res Team, Wako, Saitama 3510198, Japan

[6] Kyoto Univ, Dept Mat Chem, Nishikyo Ku, Kyoto 6158510, Japan

[7] Japan Synchrotron Radiat Res Inst, Sayo, Hyogo 6795198, Japan

[8] Spiber Inc, Tsuruoka, Yamagata 9970052, Japan

[9] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[10] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

[11] Kagoshima Univ, United Grad Sch Agr Sci, Korimoto, Kagoshima 8900065, Japan

[12] Kokugakuin Kugayama High Sch, Tokyo 1680082, Japan

[13] Saga Univ, Grad Sch Agr, Saga 8408502, Japan

[14] Univ New South Wales, Evolut & Ecol Res Ctr, Sydney, NSW 2052, Australia

[15] Univ Trento, Dept Civil Environm & Mech Engn, Via Mesiano 77, I-38123 Trento, Italy

[16] Beihang Univ, Beijing Adv Innovat Ctr Biomed Engn, Sch Mat Sci & Engn, Beijing 100191, Peoples R China

[17] Univ Sheffield, Dept Mat Sci & Engn, Nat Mat Grp, Mappin St, Sheffield S1 3JD, S Yorkshire, England

[18] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA

[19] Univ Sains Malaysia, Sch Biol Sci, George Town 11800, Malaysia

[20] Indian Inst Technol Guwahati, Dept Biosci & Bioengn, IITG, Gauhati 781039, Assam, India

[21] IITG, Ctr Nanotechnol, Gauhati 781039, Assam, India

[22] IITG, Sch Hlth Sci & Technol, Gauhati 781039, Assam, India

[23] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia

[24] Queen Mary Univ London, Sch Engn & Mat Sci, Mile End Rd, London E1 4NS, England

[25] Deakin Univ, Inst Frontier Mat, Waurn Ponds, Vic 3216, Australia

[26] Soochow Univ, Coll Text & Clothing Engn, Suzhou 215123, Peoples R China

[27] Univ New South Wales, Mark Wainwright Analyt Ctr, Sydney, NSW 2052, Australia

来源：

SCIENCE ADVANCES | 2022年 / 8卷 / 41期

关键词：

MECHANICAL-PROPERTIES; EVOLUTION; PHYLOGENY; FIBERS; GENES; CONSERVATION; DIVERSITY; SOFTWARE; FIBROIN; ORIGIN;

D O I：

10.1126/sciadv.abo6043

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.

引用

页数：14

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