Beyond Global Charge: Role of Amine Bulkiness and Protein Fingerprint on Nanoparticle-Cell Interaction

被引:17
作者
Burnand, David [1 ,2 ]
Milosevic, Ana [2 ]
Balog, Sandor [2 ]
Spuch-Calvar, Miguel [2 ]
Rothen-Rutishauser, Barbara [2 ]
Dengjel, Jorn [3 ]
Kinnear, Calum [4 ]
Moore, Thomas L. [2 ]
Petri-Fink, Alke [1 ,2 ]
机构
[1] Univ Fribourg, Chem Dept, CH-1700 Fribourg, Switzerland
[2] Univ Fribourg, Adolphe Merkle Inst, CH-1700 Fribourg, Switzerland
[3] Univ Fribourg, Dept Biol, CH-1700 Fribourg, Switzerland
[4] Univ Melbourne, Sch Chem, Parkville, Vic 3010, Australia
基金
瑞士国家科学基金会;
关键词
amine density; nanoparticle; particle-cell interaction; protein fingerprint; surface charge; POLY(VINYL ALCOHOL); SURFACE-CHARGE; IN-VITRO; INORGANIC NANOPARTICLES; REDUCTIVE METHYLATION; COLLOIDAL STABILITY; GOLD NANOPARTICLES; CORONA; SIZE; ADSORPTION;
D O I
10.1002/smll.201802088
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Amino groups presented on the surface of nanoparticles are well-known to be a predominant factor in the formation of the protein corona and subsequent cellular uptake. However, the molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type and density affect the protein corona and cellular association of gold nanoparticles with cells in vitro. Four specific poly(vinyl alcohol-co-N-vinylamine) copolymers are synthesized containing primary, secondary, or tertiary amines. Particle cellular association (i.e., cellular uptake and surface adsorption), as well as protein corona composition, are then investigated. It is found that the protein corona (as a consequence of "amine bulkiness") and amine density are both important in dictating cellular association. By evaluating the nanoparticle surface chemistry and the protein fingerprint, proteins that are significant in mediating particle-cell association are identified. In particular, primary amines, when exposed on the polymer side chain, are strongly correlated with the presence of alpha-2-HS-glycoprotein, and promote nanoparticle cellular association.
引用
收藏
页数:9
相关论文
共 46 条
[1]   Effect of Gold Nanoparticle Aggregation on Cell Uptake and Toxicity [J].
Albanese, Alexandre ;
Chan, Warren C. W. .
ACS NANO, 2011, 5 (07) :5478-5489
[2]   Alpha2-HS glycoprotein: a protein in search of a function [J].
Arnaud, P ;
Kalabay, L .
DIABETES-METABOLISM RESEARCH AND REVIEWS, 2002, 18 (04) :311-314
[3]   Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering [J].
Balog, S. ;
Rodriguez-Lorenzo, L. ;
Monnier, C. A. ;
Obiols-Rabasa, M. ;
Rothen-Rutishauser, B. ;
Schurtenberger, P. ;
Petri-Fink, A. .
NANOSCALE, 2015, 7 (14) :5991-5997
[4]   Characterization of partially hydrolyzed poly(vinyl alcohol). Effect of poly(vinyl alcohol) molecular architecture on aqueous phase conformation [J].
Budhlall, BM ;
Landfester, K ;
Sudol, ED ;
Dimonie, VL ;
Klein, A ;
El-Aasser, MS .
MACROMOLECULES, 2003, 36 (25) :9477-9484
[5]   Catechol-derivatized poly(vinyl alcohol) as a coating molecule for magnetic nanoclusters [J].
Burnand, David ;
Monnier, Christophe A. ;
Redjem, Anthony ;
Schaefer, Mark ;
Rothen-Rutishauser, Barbara ;
Kilbinger, Andreas ;
Petri-Fink, Alke .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2015, 380 :157-162
[6]   Effect of surface properties in protein corona development on mesoporous silica nanoparticles [J].
Clemments, Alden M. ;
Muniesa, Carlos ;
Landry, Christopher C. ;
Botella, Pablo .
RSC ADVANCES, 2014, 4 (55) :29134-29138
[7]   Far beyond primary poly(vinylamine)s through free radical copolymerization and amide hydrolysis [J].
Drean, Mathilde ;
Guegan, Philippe ;
Jerome, Christine ;
Rieger, Jutta ;
Debuigne, Antoine .
POLYMER CHEMISTRY, 2016, 7 (01) :69-78
[8]   Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro [J].
Fytianos, Kleanthis ;
Rodriguez-Lorenzo, Laura ;
Clift, Martin J. D. ;
Blank, Fabian ;
Vanhecke, Dimitri ;
von Garnier, Christophe ;
Petri-Fink, Alke ;
Rothen-Rutishauser, Barbara .
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2015, 11 (03) :633-644
[9]   Nanoparticles with decreasing surface hydrophobicities:: influence on plasma protein adsorption [J].
Gessner, A ;
Waicz, R ;
Lieske, A ;
Paulke, BR ;
Mäder, K ;
Müller, RH .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 196 (02) :245-249
[10]   Determination of size and concentration of gold nanoparticles from UV-Vis spectra [J].
Haiss, Wolfgang ;
Thanh, Nguyen T. K. ;
Aveyard, Jenny ;
Fernig, David G. .
ANALYTICAL CHEMISTRY, 2007, 79 (11) :4215-4221