Surface morphology of thin lysozyme films produced by matrix-assisted pulsed laser evaporation (MAPLE)

被引：13

作者：

Purice, A. ^{[1
,2
]}

Schou, J. ^{[1
]}

Pryds, N. ^{[3
]}

Filipescu, A. ^{[2
]}

Dinescu, A. ^{[2
]}

机构：

[1] Tech Univ Denmark, Riso Natl Lab, Dept Opt & Plasma REs, DK-4000 Roskilde, Denmark

[2] Natl Inst Laser, Plasma & Radiat Phys, RO-077125 Bucharest, Romania

[3] Tech Univ Denmark, Riso Natl Lab, Fuel Cells & Solid State Chem Dept, DK-4000 Roskilde, Denmark

来源：

APPLIED SURFACE SCIENCE | 2007年 / 254卷 / 04期

关键词：

thin films; MAPLE; lysozyme; SEM; AFM;

D O I：

10.1016/j.apsusc.2007.07.181

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Thin films of the protein, lysozyme, have been deposited by the matrix-assisted pulsed laser evaporation (MAPLE) technique. Frozen targets of 0.3-1.0 wt.% lysozyme dissolved in ultrapure water were irradiated by laser light at 355 mn with a fluence of 2 J/cm(2). The surface quality of the thin lysozyme films of different thickness deposited on 7 rum x 7 turn Si-(1 0 0) -wafers was investigated with scanning electron microscopy and atomic force microscopy. Already at comparatively low thickness, similar to 20 nm, the substrate is covered by intact lysozyme molecules and fragments. The concentration of lysozyme in the ice matrix apparently does not play any significant role for the morphology of the film. The morphology obtained with MAPLE has been compared with results for direct laser irradiation of a pressed lysozyme sample (i.e. pulsed laser deposition (PLD)). (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：1244 / 1248

页数：5

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