Influence of storage temperature/time for cryoprecipitate on concentration of coagulation factor VIII and pass rate

Objective: To investigate the changes in the concentration of coagulation factor VIII and the pass rate of cryoprecipitate at different storage temperatures and times. Methods: Ninety packs of 400 mL whole blood collected from January to August 2018 were prepared into 90 packs of 1U cryoprecipitate which were randomly divided into two groups (45 bags each) and stored in the refrigerator at -30 degrees C and -80 degrees C, respectively. After 3, 6, 9 and 12 months of storage, the concentration of coagulation factor VIII, activated clotting time (ACT) and time to maximum amplitude (TMA) and the pass rate of cryoprecipitate were compared. Results: Under same storage temperature, the concentration of coagulation factor VIII decreased significantly with the extension of storage time (P < 0.05); for same storage time, the concentration coagulation factor VIII at -80 degrees C was significantly higher than those at -30 degrees C (P < 0.05). Under same storage temperature, the ACT and TMA values were significantly longer with the extension of storage time (P < 0.05); for same storage time, the ACT and TMA values at -80 degrees C were shorter than those at -30 degrees C (P < 0.05). There was no significant difference in the reduction rate of concentration of coagulation factor VIII under combined influence of storage temperature and time. The difference in the pass rates of coagulation factor VIII was significant at different storage temperatures (P < 0.05) and was not significant at different times (P > 0.05). Conclusion: With the prolongation of storage time, the concentration of coagulation factor VIII will continue to decrease and the clotting time will continue to prolong. Storage temperature is the main factor affecting the concentration of coagulation factor VIII, ACT and TMA of cryoprecipitate. If the storage time is expected to exceed 6 months, ultra-low temperature storageis recommended for better quality.