Measurement of mechanical tractions exerted by cells in three-dimensional matrices

被引：311

作者：

Legant, Wesley R. ^{[1
]}

Miller, Jordan S. ^{[1
]}

Blakely, Brandon L. ^{[1
]}

Cohen, Daniel M. ^{[1
]}

Genin, Guy M. ^{[2
]}

Chen, Christopher S. ^{[1
]}

机构：

[1] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

[2] Washington Univ, Dept Mech Aerosp & Struct Engn, St Louis, MO USA

来源：

NATURE METHODS | 2010年 / 7卷 / 12期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

CYTOSKELETAL TENSION; TISSUE; SHAPE; 3RD-DIMENSION; SUBSTRATE; FORCE;

D O I：

10.1038/NMETH.1531

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative measurements of cell-generated forces have heretofore required that cells be cultured on two-dimensional substrates. We describe a technique to quantitatively measure three-dimensional traction forces exerted by cells fully encapsulated in well-defined elastic hydrogel matrices. Using this approach we measured traction forces for several cell types in various contexts and revealed patterns of force generation attributable to morphologically distinct regions of cells as they extend into the surrounding matrix.

引用

页码：969 / U113

页数：5

共 19 条

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