Mutagenic, recombinogenic and carcinogenic potential of thiamethoxam insecticide and formulated product in somatic cells of Drosophila melanogaster

被引：15
作者：
de Morais, Cassio Resende [1 ]
Carvalho, Stephan Malfitano [2 ]
Carvalho Naves, Maria Paula [1 ]
Araujo, Galber [1 ,3 ]
Alves de Rezende, Alexandre Azenha [1 ]
Bonetti, Ana Maria [1 ]
Spano, Mario Antonio [1 ]
机构：
[1] Univ Fed Uberlandia, Inst Genet & Biochem, Campus Umuarama, BR-38900402 Uberlandia, MG, Brazil
[2] Univ Fed Lavras, Dept Entomol, POB 3037, BR-37200000 Lavras, MG, Brazil
[3] Univ Salzburg, Dept Mol Biol, A-5020 Salzburg, Austria
来源：
CHEMOSPHERE | 2017年 / 187卷
关键词：
Actara; Insecticide; Neonicotinoid; SMART; Warts; BEMISIA-TABACI HEMIPTERA; RECOMBINATION TEST; DNA-DAMAGE; TUMOR-SUPPRESSOR; OXIDATIVE STRESS; HOMOLOGOUS RECOMBINATION; BIOCHEMICAL-MECHANISMS; CROSS-RESISTANCE; GENE-EXPRESSION; ALLIUM-CEPA;
D O I：
10.1016/j.chemosphere.2017.08.108
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Thiamethoxam (TMX) belongs to a class of neuro-active insecticides referred as neonicotinoids, while actara (R) (AC) is one of the most popular TMX-based products in Brazil. The aim of this study was to evaluate the mutagenic, recombinogenic and carcinogenic potential of TMX and AC insecticides. The mutagenic and recombinogenic effect of TMX and AC were evaluated in vivo by the Somatic Mutation and Recombination Test (SMART) while carcinogenic effects were evaluated through the Test for Detection of Epithelial Tumor Clones (wts test), both in somatic cells of Drosophila melanogaster. In the SMART, third instar larvae from standard (ST) and high bioactivation (HB) crosses were treated with different concentrations of TMX and AC (2.4; 4.8; 9.7 x 10(-4) mM and 1.9 x 10(-3) mM). The results revealed mutagenic effects at the highest concentrations tested in the HB cross. In the test for the detection of epithelial tumor, third instar larvae resulting from the cross between wts/TM3, Sb-1 virgin females and mwh/mwh males were treated with the same concentrations of TMX and AC used in the SMART. No carcinogenic effect was observed at any of the concentrations tested. In this work, the inhibition of the mechanism of repair by homologous recombination was observed in flies exposed to 9.7 x 10(-4) and 1.9 x 10(-3) mM of AC. In conclusion, TMX and AC demonstrated to be a promutagen in the highest concentrations tested. (C) 2017 Elsevier Ltd. All rights reserved.
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页码：163 / 172
页数：10
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