Anti-β2-glycoprotein I antibodies recognizing platelet factor 4-heparin complex in antiphospholipid syndrome in patient substantiated with mouse model

被引:8
作者
Bourhim, M
Darnige, L
Legallais, C
Arvieux, J
Cevallos, R
Pouplard, C
Vijayalakshmi, MA [1 ]
机构
[1] Univ Technol Compiegne, Lab Interact Mol & Technol Separat, F-60205 Compiegne, France
[2] Ctr Hosp Compiegne, Hematol Lab, Compiegne, France
[3] Univ Technol Compiegne, Lab Biomecan & Instrumentat Med, F-60205 Compiegne, France
[4] Ctr Hosp Brest, Immunol Lab, Brest, France
[5] Ctr Hosp Compiegne, Serv Med Interne, Compiegne, France
[6] CHU Tours, Hop Trousseau, Hematol Lab, Tours, France
关键词
anti phospholipid antibodies; antiphospholipid syndrome; beta(2)-glycoprotein I; platelet factor 4-heparin complex; heparin; anticardiolipin; crossreactivity; thrombocytopenia; heparin-induced thrombocytopenia; animal model;
D O I
10.1002/jmr.619
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The antiphospholipid syndrome is defined by the presence of antiphospholipid antibodies associated with arterial and/or venous thrombosis, and recurrent abortion accompanied often by thrombocytopenia. These antibodies are heterogeneous and react against phospholipid-binding proteins such as beta(2)-glycoprotein I (beta(2)GPI) and prothrombin. The recognition of anti-beta(2)-glycoprotein I (anti-beta(2)GPI) by platelet factor 4-heparin complex (PF4-Hc) has been previously evoked and partially confirmed by the present inhibition studies. Further, the anti-beta(2)-glycoprotein I antibodies were purified from a patient with primary antiphospholipid syndrome using Affi-gel(R)-10-beta(2)GPI immunoaffinity chromatography. The purified anti-beta(2)GPI IgM as well as patient serum equally recognized PF4-Hc in ELISA mode. In order to substantiate this data and to better understand we studied an animal model using mouse active immunization with the purified human anti-beta(2)GPI. The mice showed a significant decrease in their platelet count. In addition the ELISA responses of the immunized mice sera were positive against both beta(2)GPI and PF4-Hc, substantiating the double recognition. Despite many previous reported animal model studies, this is the first time we have shown the specific recognition of anti-beta(2)GPI antibodies by PF4-Hc, the results in the induced mice correlating the data observed with some patients. Copyright (C) 2003 John Wiley Sons, Ltd.
引用
收藏
页码:125 / 130
页数:6
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