Facile monitoring of baculovirus infection for foreign protein expression under very late polyhedrin promoter using green fluorescent protein reporter under early-to-late promoter

被引：6
|
作者：
Dalal, NG
Bentley, WE
Cha, HJ [1 ]
机构：
[1] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
[3] Univ Maryland, Ctr Biosyst Res, College Pk, MD 20742 USA
[4] Univ Maryland, Dept Chem Engn, College Pk, MD 20742 USA
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2005年 / 24卷 / 01期
关键词：
baculovirus infection; early ETL promoter; green fluorescent protein; recombinant protein production;
D O I：
10.1016/j.bej.2005.01.026
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A recombinant baculovirus derived from the Autographa californica nuclear polyhedrosis virus (AcNPV) was constructed so that the green fluorescent protein (GFP) was produced via the early-to-late (ETL) promoter. This enabled rapid monitoring of the infection of Sf-9 insect cells. Notably, GFP and its fluorescence appeared similar to 18 h prior to proteins expressed using very late polyhedrin (Polh) promoter. It is anticipated that the use of GFP under the control of ETL promoter will facilitate vector construction, virus isolation, and titer determination. (c) 2005 Elsevier B.V. All rights reserved.
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页码：27 / 30
页数：4
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