Use of RNA interference to prevent lethal murine West Nile virus infection

被引：70

作者：

Bai, FW ^{[1
]}

Wang, T ^{[1
]}

Pal, U ^{[1
]}

Bao, F ^{[1
]}

Gould, LH ^{[1
]}

Fikrig, E ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Internal Med, Rheumatol Sect,Anlyan Ctr Med Res & Educ, New Haven, CT 06520 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2005年 / 191卷 / 07期

关键词：

D O I：

10.1086/428507

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

West Nile virus causes fatal encephalitis in humans, mice, and other vertebrates. In the present study, we demonstrate that small interfering RNAs (siRNAs) inhibit West Nile virus replication in vitro. Moreover, the administration of siRNAs to mice by hydrodynamic injection 24 h before challenge with an intraperitoneal inoculum of West Nile virus reduced the viral load and afforded partial protection from lethal infection. These data show the efficacy of the prophylactic use of siRNAs against a viral infection in vivo and suggest new strategies to combat West Nile virus.

引用

页码：1148 / 1154

页数：7

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