Hemp seed oil is chosen as a feedstock for biodiesel production, methanol and KOH were used in the bio-diesel production. The properties like chemical and physical of hemp oil biodiesel are measured to inves-tigate the engine emission and performance characteristics of diesel engine. From the results, B20 blend performance is best among other fuels tested. Besides, the Brake Specific Fuel consumption (BSFC) at full load (0.3 kg/kW h) for B20 is slightly lesser than diesel fuel (0.32 kg/kW h). This indicates that the lower diesel/hemp seed oil biodiesel blends will increase the performance as well as decrease the fuel con-sumption. Considering, the emissions from exhaust exhibited that Carbon Monoxide (CO), Hydrocarbon (HC) and Nitrox oxide (NOx) were lowered for all hemp seed oil biodiesel/diesel blends. However, smoke emission increased slightly than diesel. In general, the results concluded that hemp oil biodiesel is a best alternative as well as good substitute fuel to diesel. (c) 2021 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the 3rd International Con-ference on Materials, Manufacturing and Modelling.