Establishment of Human Neural Progenitor Cells from Human Induced Pluripotent Stem Cells with Diverse Tissue Origins

被引：20

作者：

Fukusumi, Hayato ^{[1
]}

Shofuda, Tomoko ^{[2
]}

Bamba, Yohei ^{[1
,3
]}

Yamamoto, Atsuyo ^{[2
]}

Kanematsu, Daisuke ^{[1
]}

Handa, Yukako ^{[1
]}

Okita, Keisuke ^{[4
]}

Nakamura, Masaya ^{[5
]}

Yamanaka, Shinya ^{[4
]}

Okano, Hideyuki ^{[3
]}

Kanemura, Yonehiro ^{[1
,6
]}

机构：

[1] Natl Hosp Org, Osaka Natl Hosp, Inst Clin Res, Div Regenerat Med, Osaka 5400006, Japan

[2] Natl Hosp Org, Osaka Natl Hosp, Inst Clin Res, Div Stem Cell Res, Osaka 5400006, Japan

[3] Keio Univ, Sch Med, Dept Physiol, Tokyo 1608582, Japan

[4] Kyoto Univ, Ctr iPS Cell Res & Applicat CiRA, Kyoto 6068507, Japan

[5] Keio Univ, Sch Med, Dept Orthoped Surg, Tokyo 1608582, Japan

[6] Natl Hosp Org, Osaka Natl Hosp, Dept Neurosurg, Osaka 5400006, Japan

来源：

STEM CELLS INTERNATIONAL | 2016年 / 2016卷

基金：

日本科学技术振兴机构;

关键词：

IPSC-DERIVED NEURONS; SPINAL MOTOR-NEURONS; HUMAN ES; DIFFERENTIATION; LINES; PROLIFERATION; PRECURSORS; DERIVATION; INDUCTION;

D O I：

10.1155/2016/7235757

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human neural progenitor cells (hNPCs) have previously been generated from limited numbers of human induced pluripotent stem cell (hiPSC) clones. Here, 21 hiPSC clones derived from human dermal fibroblasts, cord blood cells, and peripheral blood mononuclear cells were differentiated using two neural induction methods, an embryoid body (EB) formation-based method and an EB formation method using dual SMAD inhibitors (dSMADi). Our results showed that expandable hNPCs could be generated from hiPSC clones with diverse somatic tissue origins. The established hNPCs exhibited a mid/hindbrain-type neural identity and uniform expression of neural progenitor genes.

引用

页数：10

共 35 条

[1] Characterization of human embryonic stem cell lines by the International Stem Cell Initiative [J].

Adewumi, Oluseun ;

Aflatoonian, Behrouz ;

Ahrlund-Richter, Lars ;

Amit, Michal ;

Andrews, Peter W. ;

Beighton, Gemma ;

Bello, Paul A. ;

Benvenisty, Nissim ;

Berry, Lorraine S. ;

Bevan, Simon ;

Blum, Barak ;

Brooking, Justin ;

Chen, Kevin G. ;

Choo, Andre B. H. ;

Churchill, Gary A. ;

Corbel, Marie ;

Damjanov, Ivan ;

Draper, Jon S. ;

Dvorak, Petr ;

Emanuelsson, Katarina ;

Fleck, Roland A. ;

Ford, Angela ;

Gertow, Karin ;

Gertsenstein, Marina ;

Gokhale, Paul J. ;

Hamilton, Rebecca S. ;

Hampl, Ales ;

Healy, Lyn E. ;

Hovatta, Outi ;

Hyllner, Johan ;

Imreh, Marta P. ;

Itskovitz-Eldor, Joseph ;

Jackson, Jamie ;

Johnson, Jacqueline L. ;

Jones, Mark ;

Kee, Kehkooi ;

King, Benjamin L. ;

Knowles, Barbara B. ;

Lako, Majlinda ;

Lebrin, Franck ;

Mallon, Barbara S. ;

Manning, Daisy ;

Mayshar, Yoav ;

Mckay, Ronald D. G. ;

Michalska, Anna E. ;

Mikkola, Milla ;

Mileikovsky, Masha ;

Minger, Stephen L. ;

Moore, Harry D. ;

Mummery, Christine L. .

NATURE BIOTECHNOLOGY, 2007, 25 (07) :803-816

[2] Endogenous Wnt signalling in human embryonic stem cells generates an equilibrium of distinct lineage-specified progenitors [J].

Blauwkamp, Timothy A. ;

Nigam, Shelly ;

Ardehali, Reza ;

Weissman, Irving L. ;

Nusse, Roel .

NATURE COMMUNICATIONS, 2012, 3

[3] Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling [J].

Chambers, Stuart M. ;

Fasano, Christopher A. ;

Papapetrou, Eirini P. ;

Tomishima, Mark ;

Sadelain, Michel ;

Studer, Lorenz .

NATURE BIOTECHNOLOGY, 2009, 27 (03) :275-280

[4] NeuriteQuant: An open source toolkit for high content screens of neuronal Morphogenesis [J].

Dehmelt, Leif ;

Poplawski, Gunnar ;

Hwang, Eric ;

Halpain, Shelley .

BMC NEUROSCIENCE, 2011, 12

[5] Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals [J].

Eiraku, Mototsugu ;

Watanabe, Kiichi ;

Matsuo-Takasaki, Marni ;

Kawada, Masako ;

Yonemura, Shigenobu ;

Matsumura, Michiru ;

Wataya, Takafumi ;

Nishiyama, Ayaka ;

Muguruma, Keiko ;

Sasail, Yoshiki .

CELL STEM CELL, 2008, 3 (05) :519-532

[6] Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage [J].

Elkabetz, Yechiel ;

Panagiotakos, Georgia ;

Al Shamy, George ;

Socci, Nicholas D. ;

Tabar, Viviane ;

Studer, Lorenz .

GENES & DEVELOPMENT, 2008, 22 (02) :152-165

[7] Capture of Neuroepithelial-Like Stem Cells from Pluripotent Stem Cells Provides a Versatile System for In Vitro Production of Human Neurons [J].

Falk, Anna ;

Koch, Philipp ;

Kesavan, Jaideep ;

Takashima, Yasuhiro ;

Ladewig, Julia ;

Alexander, Michael ;

Wiskow, Ole ;

Tailor, Jignesh ;

Trotter, Matthew ;

Pollard, Steven ;

Smith, Austin ;

Bruestle, Oliver .

PLOS ONE, 2012, 7 (01)

[8] Mitochondrial dysfunction associated with increased oxidative stress and α-synuclein accumulation in PARK2 iPSC-derived neurons and postmortem brain tissue [J].

Imaizumi, Yoichi ;

Okada, Yohei ;

Akamatsu, Wado ;

Koike, Masato ;

Kuzumaki, Naoko ;

Hayakawa, Hideki ;

Nihira, Tomoko ;

Kobayashi, Tetsuro ;

Ohyama, Manabu ;

Sato, Shigeto ;

Takanashi, Masashi ;

Funayama, Manabu ;

Hirayama, Akiyoshi ;

Soga, Tomoyoshi ;

Hishiki, Takako ;

Suematsu, Makoto ;

Yagi, Takuya ;

Ito, Daisuke ;

Kosakai, Arifumi ;

Hayashi, Kozo ;

Shouji, Masanobu ;

Nakanishi, Atsushi ;

Suzuki, Norihiro ;

Mizuno, Yoshikuni ;

Mizushima, Noboru ;

Amagai, Masayuki ;

Uchiyama, Yasuo ;

Mochizuki, Hideki ;

Hattori, Nobutaka ;

Okano, Hideyuki .

MOLECULAR BRAIN, 2012, 5

[9] Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity [J].

Kanemura, Y ;

Mori, H ;

Kobayashi, S ;

Islam, O ;

Kodama, E ;

Yamamoto, A ;

Nakanishi, Y ;

Arita, N ;

Yamasaki, M ;

Kano, H ;

Hara, M ;

Miyake, J .

JOURNAL OF NEUROSCIENCE RESEARCH, 2002, 69 (06) :869-879

[10] Robust Enhancement of Neural Differentiation from Human ES and iPS Cells Regardless of their Innate Difference in Differentiation Propensity [J].

Kim, Dae-Sung ;

Lee, Jae Souk ;

Leem, Joong Woo ;

Huh, Yong Jun ;

Kim, Ji Young ;

Kim, Han-Soo ;

Park, In-Hyun ;

Daley, George Q. ;

Hwang, Dong-Youn ;

Kim, Dong-Wook .

STEM CELL REVIEWS AND REPORTS, 2010, 6 (02) :270-281

← 1 2 3 4 →