Single Cell Technologies: Beyond Microfluidics

被引：13

作者：

Li, Haikuo ^{[1
,2
]}

Humphreys, Benjamin D. ^{[1
,2
]}

机构：

[1] Washington Univ, Sch Med, Div Nephrol, 660 South Euclid Ave,CB 8129, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Dev Biol, St Louis, MO 63110 USA

来源：

KIDNEY360 | 2021年 / 2卷 / 07期

基金：

美国国家卫生研究院;

关键词：

genetics; basic science; genomics; single-cell analysis; small cytoplasmic RNA; GENE-EXPRESSION; CHROMATIN ACCESSIBILITY; RNA-SEQ; TRANSCRIPTOME; IDENTIFICATION; LINEAGE; ENABLES;

D O I：

10.34067/KID.0001822021

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Single-cell RNA-sequencing (scRNA-seq) has been widely adopted in recent years due to standardized protocols and automation, reliability, and standardized bioinformatic pipelines. The most widely adopted platform is the 10x Genomics solution. Although powerful, this system is limited by its high cost, moderate throughput, and the inability to customize due to fixed kit components. This study will cover new approaches that do not rely on microfluidics and thus have low entry costs, are highly customizable, and are within the reach of any laboratory possessing molecular biology expertise.

引用

页码：1196 / 1204

页数：9

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