Pregnancy-associated diseases are characterized by the composition of the systemic regulatory T cell (Treg) pool with distinct subsets of Tregs

被引：112

作者：

Steinborn, A. ^{[1
]}

Schmitt, E. ^{[4
]}

Kisielewicz, A. ^{[1
]}

Rechenberg, S. ^{[1
]}

Seissler, N. ^{[2
]}

Mahnke, K. ^{[3
]}

Schaier, M. ^{[2
]}

Zeier, M. ^{[2
]}

Sohn, C. ^{[1
]}

机构：

[1] Heidelberg Univ, Dept Obstet & Gynecol, D-69115 Heidelberg, Germany

[2] Heidelberg Univ, Dept Nephrol, D-69115 Heidelberg, Germany

[3] Heidelberg Univ, Dept Dermatol, D-69115 Heidelberg, Germany

[4] Johannes Gutenberg Univ Mainz, Inst Immunol, D-6500 Mainz, Germany

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 2012年 / 167卷 / 01期

关键词：

pre-eclampsia; pregnancy; preterm labour; regulatory T cells; subsets of regulatory T cells; SUPPRESSIVE FUNCTION; PERIPHERAL-BLOOD; EXPRESSION; TOLERANCE; FOXP3(+); PREECLAMPSIA; REG;

D O I：

10.1111/j.1365-2249.2011.04493.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dysregulations concerning the composition and function of regulatory T cells (Tregs) are assumed to be involved in the pathophysiology of complicated pregnancies. We used six-colour flow cytometric analysis to demonstrate that the total CD4+CD127low+/-CD25+forkhead box protein 3 (FoxP3)+ Treg cell pool contains four distinct Treg subsets: DRhigh+CD45RA-, DRlow+CD45RA-, DR-CD45RA- Tregs and naive DR-CD45RA+ Tregs. During the normal course of pregnancy, the most prominent changes in the composition of the total Treg cell pool were observed between the 10th and 20th weeks of gestation, with a clear decrease in the percentage of DRhigh+CD45RA- and DRlow+CD45RA- Tregs and a clear increase in the percentage of naive DR-CD45RA+ Tregs. After that time, the composition of the total Treg cell pool did not change significantly. Its suppressive activity remained stable during normally progressing pregnancy, but decreased significantly at term. Compared to healthy pregnancies the composition of the total Treg cell pool changed in the way that its percentage of naive DR-CD45RA+ Tregs was reduced significantly in the presence of pre-eclampsia and in the presence of preterm labour necessitating preterm delivery (PL). Interestingly, its percentage of DRhigh+CD45RA- and DRlow+CD45RA- Tregs was increased significantly in pregnancies affected by pre-eclampsia, while PL was accompanied by a significantly increased percentage of DR-CD45RA- and DRlow+CD45RA- Tregs. The suppressive activity of the total Treg cell pool was diminished in both patient collectives. Hence, our findings propose that pre-eclampsia and PL are characterized by homeostatic changes in the composition of the total Treg pool with distinct Treg subsets that were accompanied by a significant decrease of its suppressive activity.

引用

页码：84 / 98

页数：15

共 32 条

[1] The role of regulatory T cells in alloantigen tolerance [J].

Aluvihare, Varuna R. ;

Betz, Alexander G. .

IMMUNOLOGICAL REVIEWS, 2006, 212 :330-343

[2] Tolerance, suppression and the fetal allograft [J].

Aluvihare, VR ;

Kallikourdis, M ;

Betz, AG .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2005, 83 (02) :88-96

[3] Regulatory T cells mediate maternal tolerance to the fetus [J].

Aluvihare, VR ;

Kallikourdis, M ;

Betz, AG .

NATURE IMMUNOLOGY, 2004, 5 (03) :266-271

[4] Expansion of CD4+CD25+ and FOXP3+ regulatory T cells during the follicular phase of the menstrual cycle:: Implications for human reproduction [J].

Arruvito, Lourdes ;

Sanz, Marianela ;

Banham, Alison H. ;

Fainboim, Leonardo .

JOURNAL OF IMMUNOLOGY, 2007, 178 (04) :2572-2578

[5] Cutting Edge: Responder T Cells Regulate Human DR+ Effector Regulatory T Cell Activity via Granzyme B [J].

Ashley, Charles W. ;

Baecher-Allan, Clare .

JOURNAL OF IMMUNOLOGY, 2009, 183 (08) :4843-4847

[6] MHC class II expression identifies functionally distinct human regulatory T cells [J].

Baecher-Allan, Clare ;

Wolf, Elizabeth ;

Haller, David A. .

JOURNAL OF IMMUNOLOGY, 2006, 176 (08) :4622-4631

[7] FOXP3+ regulatory T cells:: Current controversies and future perspectives [J].

Banham, Alison H. ;

Powrie, Fiona M. ;

Suri-Payer, Elisabeth .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2006, 36 (11) :2832-2836

[8]

Bianchi D W, 2003, J Matern Fetal Neonatal Med, V14, P123, DOI 10.1080/713606541

[9] Different Proliferative Potential and Migratory Characteristics of Human CD4+ Regulatory T Cells That Express either CD45RA or CD45R0 [J].

Booth, Nicola J. ;

McQuaid, Arthur J. ;

Sobande, Toni ;

Kissane, Steve ;

Agius, Elaine ;

Jackson, Sarah E. ;

Salmon, Mike ;

Falciani, Francesco ;

Yong, Kwee ;

Rustin, Malcolm H. ;

Akbar, Arne N. ;

Vukmanovic-Stejic, Milica .

JOURNAL OF IMMUNOLOGY, 2010, 184 (08) :4317-4326

[10] The role of placental Fas ligand in maintaining immune privilege at maternal-fetal interfaces [J].

Guller, S ;

LaChapelle, L .

SEMINARS IN REPRODUCTIVE ENDOCRINOLOGY, 1999, 17 (01) :39-44

← 1 2 3 4 →