Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically

被引：65

作者：

Ringrose, Jeffrey H. ^{[1
,2
]}

van Solinge, Wouter W. ^{[3
]}

Mohammed, Shabaz ^{[1
,2
]}

O'Flaherty, Martina C. ^{[1
,2
]}

van Wijk, Richard ^{[3
]}

Heck, Albert J. R. ^{[1
,2
]}

Slijper, Monique ^{[1
,2
]}

机构：

[1] Univ Utrecht, Biomol Mass Spectrometry & Prote Grp, Bijuoet Ctr Biomol Res, NL-3584 CA Utrecht, Netherlands

[2] Univ Utrecht, Utrecht Inst Pharmaceut Sci, NL-3584 CA Utrecht, Netherlands

[3] Univ Med Ctr Utrecht, Dept Clin Chem & Hematol, Lab Red Blood Cell Res, Utrecht, Netherlands

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 07期

关键词：

human erythrocyte proteome; hemoglobin depletion; carbonic anhydrase-1 depletion; erythrocyte disorder proteomics; IEX-chromatography; Ni(II) chromatography; multi-dimensional liquid chromatography;

D O I：

10.1021/pr8001029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood.

引用

页码：3060 / 3063

页数：4

共 21 条

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