Field-based evaluation of a reagent strip test for diagnosis of schistosomiasis mansoni by detecting circulating cathodic antigen (CCA) in urine in low endemic area in Ethiopia

被引:32
作者
Legesse, M. [1 ]
Erko, B. [1 ]
机构
[1] Univ Addis Ababa, Aklilu Lemma Inst Pathobiol, Addis Ababa, Ethiopia
关键词
S; mansoni; CCA; urine; reagent strip test; Ethiopia;
D O I
10.1051/parasite/2008152151
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The sensitivity, specificity, positive end negative predictive values of a reagent strip test for the diagnosis of schistosomiasis mansoni by detecting circulating cathodic antigen (CCA) in urine were evaluated using 184 stool and urine samples collected from schoolchildren living in relatively low endemic area of schistosomiasis mansoni in Ethiopia, A combined result of stool samples processed by Kato and formol ether concentration methods was used as god standard. The results showed that detection of CCA in urine using reagent strip test was slightly higher than the combined results of the stool techniques (65.2 % vs 42.4 %, p > 0.05) in suggesting the prevalence of the disease. The sensitivity, specificity, positive and negative predictive values of the reagent strip test were 76.9 %, 43.4 %, 50 % and 71.9 %, respectively. The result of egg counts using Kato method suggested that detection of urine CCA could be used to indicate the intensity of infection. Nevertheless, like that of stool examination, the reagent strip test was found to be less sensitive in case of bight to moderate infections. About 23.1 % of the study children who were excreting the eggs of the parasite were found negative by the reagent strip test. The relative insensitivity of a reagent strip test in low intensity of infection necessitates for the development of more sensitive assay that can truly discriminate schistosome-infected from nor-infected individuals.
引用
收藏
页码:151 / 155
页数:5
相关论文
共 24 条
[1]   Circulating anodic and cathodic antigen in serum and urine of mixed Schistosoma haematobium and S-mansoni infections in Office du Niger, Mali [J].
DeClercq, D ;
Sacko, M ;
Vercruysse, J ;
vandenBussche, V ;
Landoure, A ;
Diarra, A ;
Gryseels, B ;
Deelder, A .
TROPICAL MEDICINE & INTERNATIONAL HEALTH, 1997, 2 (07) :680-685
[2]  
DEELDER A, 1989, AM J TROP MED HYG, V38, P348
[3]   UNDERESTIMATION OF SCHISTOSOMA-MANSONI PREVALENCES [J].
DEVLAS, SJ ;
GRYSEELS, B .
PARASITOLOGY TODAY, 1992, 8 (08) :274-277
[4]   SEROEPIDEMIOLOGY AND SERODIAGNOSIS OF SCHISTOSOMIASIS IN KENYA USING CRUDE AND PURIFIED EGG ANTIGENS OF SCHISTOSOMA-MANSONI IN ELISA [J].
DOENHOFF, MJ ;
BUTTERWORTH, AE ;
HAYES, RJ ;
STURROCK, RF ;
OUMA, JH ;
KOECH, D ;
PRENTICE, M ;
BAIN, J .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1993, 87 (01) :42-48
[5]   Evaluation of the Kato-Katz thick smear and formol ether sedimentation techniques for quantitative diagnosis of Schistosoma mansoni infection [J].
Ebrahim, A ;
El-Morshedy, H ;
Omer, E ;
El-Daly, S ;
Barakat, R .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 57 (06) :706-708
[6]   Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications [J].
Engels, D ;
Sinzinkayo, E ;
Gryseels, B .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 54 (04) :319-324
[7]   Implication of Papio anubis in the transmission of intestinal schistosomiasis in three new foci in Kime area, Ethiopia [J].
Erko, B ;
Gebre-Michael, T ;
Balcha, F ;
Gundersen, SG .
PARASITOLOGY INTERNATIONAL, 2001, 50 (04) :259-266
[8]   EVALUATION OF AN ELISA FOR COMBINED MEASUREMENT OF CAA AND CCA IN SCHISTOSOMIASIS-MANSONI [J].
FILLIE, YE ;
VANLIESHOUT, L ;
KORNELIS, D ;
DEELDER, AM .
ACTA TROPICA, 1994, 57 (04) :279-287
[9]   Worm burdens in schistosome infections [J].
Gryseels, B ;
deVlas, SJ .
PARASITOLOGY TODAY, 1996, 12 (03) :115-119
[10]   Periodate-sensitive immunological cross-reactivity between keyhole limpet haemocyanin (KLH) and serodiagnostic Schistosoma mansoni egg antigens [J].
Hamilton, JV ;
Chiodini, PL ;
Fallon, PG ;
Doenhoff, MJ .
PARASITOLOGY, 1999, 118 :83-89