Generation and Characterization of the Monoclonal Antibody Panel Specific to the NS1 Protein of the Influenza A Virus

被引：2

作者：

Krivitskaya, V. Z. ^{[1
]}

Sorokin, E. V. ^{[1
]}

Tsareva, T. R. ^{[1
]}

Sergeeva, M. V. ^{[1
]}

Kadyrova, R. A. ^{[1
]}

Romanovskaya-Roman'ko, E. A. ^{[1
]}

Shaldzhyan, A. A. ^{[1
]}

Petrov, S. V. ^{[1
]}

Petrova, E. R. ^{[1
]}

Konovalova, N. I. ^{[1
]}

Petrova, P. A. ^{[1
]}

机构：

[1] Minist Hlth Care Russian Federat NII Grippa, Res Inst Influenza, St Petersburg 197376, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2018年 / 54卷 / 07期

关键词：

antigenic and genetic variability of NS1; NS1; protein; influenza virus; microcultural ELISA; monoclonal antibodies; REPLICATION;

D O I：

10.1134/S0003683818070049

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The paper reports on the production of a panel of seven new monoclonal antibodies (MAbs) capable of specifically binding to the NS1(1-124) fragment of the influenza virus A/Puerto Rico/8/1934 (H1N1) NS1 protein in indirect enzyme-linked immunosorbent assay (ELISA), as well as to the full-size native NS1 protein in cells infected with different subtypes of the influenza A virus (A(H1N1), A(H1N1)pdm09, A(H2N2), A(H3N2), and A(H9N2)). A sensitive variant of the microcultural ELISA based on the use of the generated MAbs is proposed; it may be utilized to detect NS1 protein in infected cells and to study its antigenic variability. The results of the performed microcultural ELISA demonstrate type- and subtype-specific antigenic variability of the influenza virus NS1 protein.

引用

页码：756 / 765

页数：10

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