Applications of direct detection device in transmission electron microscopy

被引：56

作者：

Jin, Liang ^{[1
]}

Milazzo, Anna-Clare ^{[1
]}

Kleinfelder, Stuart ^{[2
]}

Li, Shengdong ^{[2
]}

Leblanc, Philippe ^{[1
]}

Duttweiler, Fred ^{[1
]}

Bouwer, James C. ^{[1
]}

Peltier, Steven T. ^{[1
]}

Ellisman, Mark H. ^{[1
]}

Xuong, Nguyen-Huu ^{[1
]}

机构：

[1] Univ Calif San Diego, La Jolla, CA 92093 USA

[2] Univ Calif Irvine, Irvine, CA 92697 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2008年 / 161卷 / 03期

关键词：

direct detection device; active pixel sensor; image processing algorithm; electron tomography; cryo-electron microscopy; cryo-electron tomography;

D O I：

10.1016/j.jsb.2007.10.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A prototype direct detection device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120-400 keV beam energies (Xuong et al., 2007. Methods in Cell Biology, 79, 721-739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5 mu m). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：352 / 358

页数：7

共 13 条

[1] Accuracy of a simple method for deriving the presampled modulation transfer function of a digital radiographic system from an edge image [J].