Expression of the Insulin-like Growth Factor 1 (IGF-1) and Type I IGF Receptor mRNAs in Human HLE-B3 Lens Epithelial Cells

被引：0

作者：

Moschos, Marilita M. ^{[1
,2
]}

Armakolas, Athanasios ^{[1
]}

Philippou, Anastasios ^{[1
]}

Pissimissis, Nikolaos ^{[1
]}

Panteleakou, Zacharoula ^{[1
]}

Nezos, Adrianos ^{[1
]}

Kaparelou, Maria ^{[1
]}

Koutsilieris, Michael ^{[1
]}

机构：

[1] Univ Athens, Sch Med, Dept Expt Physiol, Athens 11528, Greece

[2] Univ Athens, Sch Med, Dept Ophalmol, Athens 11528, Greece

来源：

IN VIVO | 2011年 / 25卷 / 02期

关键词：

IGF-1; lens epithelium cells; IGF-1R; E-PEPTIDE ACTIONS; POSTERIOR CAPSULE OPACIFICATION; SKELETAL-MUSCLE; GENE-EXPRESSION; DIFFERENTIATION; EXERCISE; YOUNG;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background/Aim: The E peptide of the IGF-1Ec transcript has been documented to stimulate the growth of different cell lines, via a type 1 IGF-1 receptor (IGF-1R)-independent mechanism. The aim of the present study was to determine the implication of the IGF-1Ec isoform into the posterior capsule pacification process in human lens epithelium. Materials and Methods: The expression of the IGF-1 system was characterized in human HLE-B3 lens epithelium cells and the mitogenic activity of IGF-1 and synthetic E peptide and the effects of growth hormone (GH) and dihydrotestosterone (DHT) were examined, using qualitative real-time PCR, RT-PCR, Western blot analysis and trypan blue exclusion assays in wild-type and IGF-1R knockout HLE-B3 cells. Results: The data showed that HLE-B3 cells express only the IGF-1Ea and IGF-1R transcripts. GH increased the expression of IGF-1 Ea and of the previously undetectable IGF-1Eb mRNA. Finally, IGF-1 did not present any activity in the knock-out cells. Conclusion: The IGF-1Ea isoform is the main source for the formation of mature IGF-1 in HLE-B3 cells. The effects of exogenous IGF-1 depend on the existence of IGF-1R. IGF-1 Ec is not expressed even in the presence of GH or DHT nor has it any effect on cell proliferation.

引用

页码：179 / 184

页数：6

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