Identification of p53-dependent genes potentially involved in UVB-mediated premature senescence of human skin fibroblasts using siRNA technology

被引：18

作者：

Borlon, Celine ^{[1
]}

Vankoningsloo, Sebastien ^{[1
]}

Godard, Patrice ^{[1
]}

Debacq-Chainiaux, Florence ^{[1
]}

Toussaint, Olivier ^{[1
]}

机构：

[1] Univ Namur FUNDP, Fac Sci, Dept Biol, Res Unit Cellular Biol URBC, B-5000 Namur, Belgium

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2008年 / 129卷 / 03期

关键词：

senescence; fibroblasts; UVB; p53; gene expression; DNA array;

D O I：

10.1016/j.mad.2007.10.014

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Premature senescence of skin human diploid fibroblasts is induced after a series of 10 sublethal exposures to UVB at 2.5 kJ/m(2) with appearance of several biomarkers of cellular senescence like senescence-associated P-galactosidase activity (SA beta-gal) and cell cycle arrest. Herein it is shown that the induction of UVB-induced premature senescence is associated with a transient increase of protein abundance and DNA-binding activity of p53. Silencing p53 expression with small interfering RNA (siRNA) affected the basal level of SA beta-gal and proliferative potential, but did not prevent UVB-induced increase of SA beta-gal and decrease of DNA synthesis. We used a senescence-specific low-density DNA array and p53 siRNA to study the mRNA abundance of 240 senescence-related genes and identified several potential p53-dependent genes differentially expressed after the repeated exposures to UVB. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

引用

页码：109 / 119

页数：11

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