Curdione ameliorates bleomycin-induced pulmonary fibrosis by repressing TGF-β-induced fibroblast to myofibroblast differentiation

被引：77

作者：

Liu, Peng ^{[1
]}

Miao, Kang ^{[1
]}

Zhang, Lei ^{[1
]}

Mou, Yong ^{[1
]}

Xu, Yongjian ^{[1
]}

Xiong, Weining ^{[1
,2
]}

Yu, Jun ^{[3
]}

Wang, Yi ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Resp & Crit Care Med, Key Lab Pulm Dis,Tongji Hosp,Hlth Minist, Key Cite Natl Clin Res Ctr Resp Dis,Wuhan Clin Me, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[2] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Resp Med, Sch Med, 639 Zhizaoju Lu, Shanghai 201999, Peoples R China

[3] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Thorac Surg, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

来源：

RESPIRATORY RESEARCH | 2020年 / 21卷 / 01期

基金：

中国国家自然科学基金;

关键词：

IPF; Fibroblast; Myofibroblast; Curdione; TGF-beta; 1; PROTECTS MICE; PROLIFERATION;

D O I：

10.1186/s12931-020-1300-y

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Background Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible disease characterized by excessive fibroblast to myofibroblast differentiation with limited therapeutic options. Curdione, a sesquiterpene compound extracted from the essential oil of Curcuma aromatica Salisb, has anti-inflammatory and anti-tumor effects. However, the role of curdione in IPF is still unclear. Methods The effects of curdione were evaluated in a bleomycin (BLM)-induced pulmonary fibrosis mouse model. C57BL/6 mice were treated with BLM on day 0 by intratracheal injection and intraperitoneal administered curdione or vehicle. In vitro study, expression of fibrotic protein was examined and the transforming growth factor (TGF)-beta-related signaling was evaluated in human pulmonary fibroblasts (HPFs) treated with curdione following TGF-beta 1 stimulation. Results Histological and immunofluorescent examination showed that curdione alleviated BLM-induced lung injury and fibrosis. Specifically, curdione significantly attenuated fibroblast to myofibroblast differentiation in the lung in BLM induced mice. Furthermore, curdione also decreased TGF-beta 1 induced fibroblast to myofibroblast differentiation in vitro, as evidenced by low expression of alpha-SMA, collagen 1 and fibronectin in a dose dependent manner. Mechanistically, curdione suppressed the phosphorylation of Smad3 following TGF-beta 1 treatment, thereby inhibiting fibroblast differentiation. Conclusions Overall, curdione exerted therapeutic effects against pulmonary fibrosis via attenuating fibroblast to myofibroblast differentiation. As curdione had been shown to be safe and well-tolerated in BLM-induced mouse model, curdione might be useful for developing novel therapeutics for IPF.

引用

页数：10

共 50 条

[21] ACPA Alleviates Bleomycin-Induced Pulmonary Fibrosis by Inhibiting TGF-β-Smad2/3 Signaling-Mediated Lung Fibroblast Activation [J].

Chen, Dongxin ;

Tang, Huirong ;

Jiang, Hongchao ;

Sun, Lei ;

Zhao, Wenjuan ;

Qian, Feng .

FRONTIERS IN PHARMACOLOGY, 2022, 13

[22] Baricitinib Attenuates Bleomycin-Induced Pulmonary Fibrosis in Mice by Inhibiting TGF-β1 Signaling Pathway [J].

Gu, Songtao ;

Liang, Jingjing ;

Zhang, Jianwei ;

Liu, Zhichao ;

Miao, Yang ;

Wei, Yuli ;

Li, Shimeng ;

Gu, Jinying ;

Cui, Yunyao ;

Xiao, Ting ;

Li, Xiaohe ;

Yang, Cheng .

MOLECULES, 2023, 28 (05)

[23] Grape seed extract ameliorates bleomycin-induced mouse pulmonary fibrosis [J].

Liu, Qi ;

Jiang, Jun-xia ;

Liu, Ya-nan ;

Ge, Ling-tian ;

Guan, Yan ;

Zhao, Wei ;

Jia, Yong-liang ;

Dong, Xin-wei ;

Sun, Yun ;

Xie, Qiang-min .

TOXICOLOGY LETTERS, 2017, 273 :1-9

[24] Lanatoside C protects mice against bleomycin-induced pulmonary fibrosis through suppression of fibroblast proliferation and differentiation [J].

Nie, Yunjuan ;

Zhang, Dan ;

Jin, Zhewu ;

Li, Boyu ;

Wang, Xue ;

Che, Huilian ;

You, Yaqian ;

Qian, Xiaohang ;

Zhang, Yang ;

Zhao, Peng ;

Chai, Gaoshang .

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2019, 46 (06) :575-586

[25] Neohesperidin inhibits TGF-β1/Smad3 signaling and alleviates bleomycin-induced pulmonary fibrosis in mice [J].

Guo, Jiasen ;

Fang, Yinshan ;

Jiang, Fangxin ;

Li, Lian ;

Zhou, Honggang ;

Xu, Xiaojun ;

Ning, Wen .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2019, 864

[26] Heat Shock Protein 27 Plays a Pivotal Role in Myofibroblast Differentiation and in the Development of Bleomycin-Induced Pulmonary Fibrosis [J].

Park, Ah-Mee ;

Kanai, Kyosuke ;

Itoh, Tatsuki ;

Sato, Takao ;

Tsukui, Tatsuya ;

Inagaki, Yutaka ;

Selman, Moises ;

Matsushima, Kouji ;

Yoshie, Osamu .

PLOS ONE, 2016, 11 (02)

[27] Danshensu alleviates bleomycin-induced pulmonary fibrosis by inhibiting lung fibroblast-to-myofibroblast transition via the MEK/ERK signaling pathway [J].

Liu, Huaman ;

Zhang, Xinyue ;

Shao, Yumeng ;

Lin, Xuehong ;

Dong, Feng ;

Liu, Xue .

BIOENGINEERED, 2021, 12 (01) :3113-3124

[28] Bergenin attenuates bleomycin-induced pulmonary fibrosis in mice via inhibiting TGF-β1 signaling pathway [J].

Li, Xiaohe ;

Wang, Yanhua ;

Liang, Jingjing ;

Bi, Zhun ;

Ruan, Hao ;

Cui, Yunyao ;

Ma, Ling ;

Wei, Yuli ;

Zhou, Bingchen ;

Zhang, Liang ;

Zhou, Honggang ;

Yang, Cheng .

PHYTOTHERAPY RESEARCH, 2021, 35 (10) :5808-5822

[29] Silencing FHL2 inhibits bleomycin-induced pulmonary fibrosis through the TGF-β1/Smad signaling pathway [J].

Shi, Mengkun ;

Cui, Huixia ;

Shi, Jialun ;

Mei, Yunqing .

EXPERIMENTAL CELL RESEARCH, 2023, 423 (02)

[30] Psoralen attenuates bleomycin-induced pulmonary fibrosis in mice through inhibiting myofibroblast activation and collagen deposition [J].

Du, Ming-Yuan ;

Duan, Jia-Xi ;

Zhang, Chen-Yu ;

Yang, Hui-Hui ;

Guan, Xin-Xin ;

Zhong, Wen-Jing ;

Liu, Yan-Zhe ;

Li, Zi-Ming ;

Cheng, Yu-Rui ;

Zhou, Yong ;

Guan, Cha-Xiang .

CELL BIOLOGY INTERNATIONAL, 2020, 44 (01) :98-107

← 1 2 3 4 5 →