Determination of tetrodotoxin and its analogs in the puffer fish Takifugu oblongus from Bangladesh by hydrophilic interaction chromatography and mass-spectrometric detection

被引：43
作者：
Diener, Marc
Christian, Bernd
Ahmed, M. Sagir
Luckas, Bernd
机构：
[1] Univ Jena, Inst Nutr, D-07743 Jena, Germany
[2] Univ Dhaka, Dept Zool, Lab Aquat Resource Management, Dhaka 1000, Bangladesh
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 389卷 / 06期
关键词：
hydrophilic interaction chromatography; liquid chromatography-tandem mass spectrometry; Takifugu oblongus; tetrodotoxin; zwitterionic hydrophilic interaction chromatography column;
D O I：
10.1007/s00216-007-1602-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Tetrodotoxin (TTX) and its analogs (TTXs), widely distributed among marine as well as terrestrial animals, induce dangerous intoxications. These highly potential toxins are also known as the causative agent of puffer fish poisoning. A newly developed highly sensitive method for determination of TTXs based on hydrophilic interaction chromatography and mass-spectrometric detection is presented. TTX, anhydrotetrodotoxin, 11-deoxytetrodotoxin and trideoxytetrodotoxin were determined in separated tissues of Bangladeshi marine puffers, Takifugu oblongus. TTX was predominant in skin, muscle and liver, whereas trideoxytetrodotoxin preponderated in the ovary. The toxicity of the various tissues was determined by a mouse bioassay.
引用
收藏
页码：1997 / 2002
页数：6
相关论文
共 27 条
[21]   NEW TETRODOTOXIN ANALOGS FROM THE NEWT CYNOPS-ENSICAUDA [J].
YASUMOTO, T ;
YOTSU, M ;
MURATA, M ;
NAOKI, H .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (07) :2344-2345
[22]   ISOLATION AND STRUCTURAL ASSIGNMENT OF 11-NORTETRODOTOXIN-6(S)-OL FROM THE PUFFER AROTHRON-NIGROPUNCTATUS [J].
YOTSU, M ;
HAYASHI, Y ;
KHORA, SS ;
SATO, S ;
YASUMOTO, T .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (02) :370-371
[23]   Identification of 4-S-Cysteinyltetrodotoxin from the liver of the puffer fish, Fugu pardalis, and formation of thiol adducts of tetrodotoxin from 4,9-anhydrotetrodotoxin [J].
Yotsu-Yamashita, M ;
Goto, A ;
Nakagawa, T .
CHEMICAL RESEARCH IN TOXICOLOGY, 2005, 18 (05) :865-871
[24]   Chemistry of puffer fish toxin [J].
Yotsu-Yamashita, M .
JOURNAL OF TOXICOLOGY-TOXIN REVIEWS, 2001, 20 (01) :51-66
[25]   Isolation and structural assignment of 5-deoxytetrodotoxin from the puffer fish Fugu poecilonotus [J].
Yotsu-Yamashita, M ;
Schimmele, B ;
Yasumoto, T .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1999, 63 (05) :961-963
[26]   5,6,11-trideoxytetrodotoxin from the puffer fish, Fugu poecilonotus [J].
YotsuYamashita, M ;
Yamagishi, Y ;
Yasumoto, T .
TETRAHEDRON LETTERS, 1995, 36 (51) :9329-9332
[27]   Occurrence of paralytic shellfish poison in Bangladeshi freshwater puffers [J].
Zaman, L ;
Arakawa, O ;
Shimosu, A ;
Onoue, Y .
TOXICON, 1997, 35 (03) :423-431
123