Whole-genome DNA methylation profiling using MethylCap-seq

被引：177

作者：

Brinkman, Arie B. ^{[1
]}

Simmer, Femke ^{[1
]}

Ma, Kelong ^{[2
]}

Kaan, Anita ^{[1
]}

Zhu, Jingde ^{[2
]}

Stunnenberg, Hendrik G. ^{[1
]}

机构：

[1] Radboud Univ Nijmegen, Dept Mol Biol, Nijmegen Ctr Mol Life Sci, Fac Sci, NL-6500 HB Nijmegen, Netherlands

[2] Shanghai Jiao Tong Univ, Canc Epigenet & Gene Therapy Grp, Shanghai Canc Inst, Shanghai, Peoples R China

来源：

METHODS | 2010年 / 52卷 / 03期

关键词：

MethylCap; DNA methylation; Genome-wide; MBD; Capture; PROMOTER DNA; CELLS;

D O I：

10.1016/j.ymeth.2010.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MethylCap-seq is a robust procedure for genome-wide profiling of DNA methylation. The approach consists of the capture of methylated DNA using the MBD domain of MeCP2, and subsequent next-generation sequencing of eluted DNA. Elution of the captured methylated DNA is done in steps using a salt gradient, which stratifies the genome into fractions with different CpG density. The enrichment reached within the individual eluates allows for cost-effective deep sequence coverage. The profiles together yield a detailed genome-wide map of methylated regions and readily allows detection of DNA methylation in known and novel regions. Here, we describe principles and details of the MethylCap-seq procedure using different sources of starting material. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：232 / 236

页数：5

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