Increasing prevalence of high-level gentamicin resistance among enterococci isolated in Greece

被引：12

作者：

Tsakris, A ^{[1
]}

Pournaras, S

Maniatis, AN

Douboyas, J

Antoniadis, A

机构：

[1] Aristotelian Univ Salonika, Sch Med, Dept Microbiol, GR-54006 Salonika, Greece

[2] AHEPA, Univ Hosp, Dept Microbiol, Salonika, Greece

[3] Univ Thessalia, Sch Med, Dept Microbiol, Larissa, Greece

来源：

CHEMOTHERAPY | 2001年 / 47卷 / 02期

关键词：

enterococci; high-level gentamicin resistance; aac(6 ')-aph(2 ''); PCR; Greece;

D O I：

10.1159/000048505

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

High-level gentamicin-resistant enterococci (HLGRE) (MIC >512 mug/ml) were frequently recovered in the AHEPA University Hospital, Thessaloniki, Greece, during a 15-month period (67/158, 42.4%). PCR testing showed that high-level gentamicin resistance was attributable to the presence of the aac(6')-aph(2") gene, while the recently described genes aph (2")-lc and aph(2")-ld were not detected. High-level gentamicin resistance could be transferred with varying frequency from 41 of 57 Enterococcus faecalis and 5 of 10 Enterococcus faecium isolates. All transconjugants acquired high-level resistance to streptomycin. HLGRE cause therapeutic problems in our region, since none of the clinically available aminoglycosides can be used in severe infections. Copyright (C) 2001 S. Karger AG, Basel.

引用

页码：86 / 89

页数：4

共 21 条

[1] Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci [J].

Chen, YS ;

Marshall, SA ;

Winokur, PL ;

Coffman, SL ;

Wilke, WW ;

Murray, PR ;

Spiegel, CA ;

Pfaller, MA ;

Doern, GV ;

Jones, RN .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (10) :2996-3001

[2] In vitro susceptibility and molecular analysis of gentamicin-resistant enterococci [J].

Chow, JW ;

Donabedian, SM ;

Clewell, DB ;

Sahm, DF ;

Zervos, MJ .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1998, 32 (03) :141-146

[3] A novel gentamicin resistance gene in Enterococcus [J].

Chow, JW ;

Zervos, MJ ;

Lerner, SA ;

Thal, LA ;

Donabedian, SM ;

Jaworski, DD ;

Tsai, S ;

Shaw, KJ ;

Clewell, DB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (03) :511-514

[4] STREPTOCOCCUS-FAECALIS SEX-PHEROMONE (CAM373) ALSO PRODUCED BY STAPHYLOCOCCUS-AUREUS AND IDENTIFICATION OF A CONJUGATIVE TRANSPOSON (TN918) [J].

CLEWELL, DB ;

AN, FY ;

WHITE, BA ;

GAWRONBURKE, C .

JOURNAL OF BACTERIOLOGY, 1985, 162 (03) :1212-1220

[5] PREVALENCE OF ANTIBIOTIC-RESISTANCE AMONG ENTEROCOCCI ISOLATED IN GREECE [J].

DOUBOYAS, J ;

TSAKRIS, A .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1995, 35 (01) :237-238

[6] IDENTIFICATION OF ENTEROCOCCUS SPECIES ISOLATED FROM HUMAN INFECTIONS BY A CONVENTIONAL TEST SCHEME [J].

FACKLAM, RR ;

COLLINS, MD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (04) :731-734

[7] NUCLEOTIDE-SEQUENCE ANALYSIS OF THE GENE SPECIFYING THE BIFUNCTIONAL 6'-AMINOGLYCOSIDE ACETYLTRANSFERASE 2''-AMINOGLYCOSIDE PHOSPHOTRANSFERASE ENZYME IN STREPTOCOCCUS-FAECALIS AND IDENTIFICATION AND CLONING OF GENE REGIONS SPECIFYING THE 2 ACTIVITIES [J].

FERRETTI, JJ ;

GILMORE, KS ;

COURVALIN, P .

JOURNAL OF BACTERIOLOGY, 1986, 167 (02) :631-638

[8] BACTEREMIA CAUSED BY HEMOLYTIC, HIGH-LEVEL GENTAMICIN-RESISTANT ENTEROCOCCUS-FAECALIS [J].

HUYCKE, MM ;

SPIEGEL, CA ;

GILMORE, MS .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (08) :1626-1634

[9] EMERGING MULTIPLY RESISTANT ENTEROCOCCI AMONG CLINICAL ISOLATES .1. PREVALENCE DATA FROM 97 MEDICAL-CENTER SURVEILLANCE STUDY IN THE UNITED-STATES [J].

JONES, RN ;

SADER, HS ;

ERWIN, ME ;

ANDERSON, SC ;

ALDRIDGE, KA ;

ALLEN, S ;

ANHALT, J ;

APPELBAUM, P ;

ARRINGTON, KL ;

AYERS, L ;

BAKER, C ;

BEAVIS, K ;

BERGER, J ;

BERTHOLD, G ;

BIRNBAUM, M ;

BOYLE, J ;

BRECHER, S ;

BRECKENRIDGE, R ;

BROWN, W ;

BRUCKNER, D ;

CARROLL, K ;

CHAUDHARY, S ;

CLEARY, T ;

COCKERILL, F ;

COYLE, M ;

CRAWFORD, V ;

DALTON, H ;

DOERN, G ;

EDBERG, S ;

GELFAND, M ;

GERLACH, EH ;

GOODMAN, N ;

GORZYNSKI, E ;

GREEN, P ;

GROSCHEL, D ;

HANFF, P ;

HANNA, B ;

HARRELL, L ;

HAUGEN, T ;

HEAGREY, M ;

HUMPHRIES, J ;

ISENBERG, H ;

JENKINS, S ;

JONES, E ;

JORGENSEN, J ;

KAUFFMAN, C ;

KEISER, J ;

KOCKA, F ;

KOMINOS, S ;

LEVISON, M .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1995, 21 (02) :85-93

[10] RESISTANCE OF ENTEROCOCCI TO AMINOGLYCOSIDES AND GLYCOPEPTIDES [J].

LECLERCQ, R ;

DUTKAMALEN, S ;

BRISSONNOEL, A ;

MOLINAS, C ;

DERLOT, E ;

ARTHUR, M ;

DUVAL, J ;

COURVALIN, P .

CLINICAL INFECTIOUS DISEASES, 1992, 15 (03) :495-501

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