Bioconversion of oxygen-pretreated Kraft lignin to microbial lipid with oleaginous Rhodococcus opacus DSM 1069

被引：104

作者：

Wei, Zhen ^{[1
,2
]}

Zeng, Guangming ^{[1
,2
]}

Huang, Fang ^{[3
]}

Kosa, Matyas ^{[3
]}

Huang, Danlian ^{[1
,2
]}

Ragauskas, Arthur J. ^{[3
,4
]}

机构：

[1] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China

[2] Hunan Univ, Minist Educ, Key Lab Environm Biol & Pollut Control, Changsha 410082, Hunan, Peoples R China

[3] Georgia Inst Technol, Inst Paper Sci & Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[4] Univ Tennessee, Dept Forestry Fisheries & Wildlife, Dept Chem & Biomol Engn, Knoxville, TN 37996 USA

来源：

GREEN CHEMISTRY | 2015年 / 17卷 / 05期

基金：

中国国家自然科学基金;

关键词：

OIL PRODUCTION; DELIGNIFICATION; DEGRADATION;

D O I：

10.1039/c5gc00422e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Kraft lignin (KL) from black liquor is an abundantly available, inexpensive aromatic resource that is regarded as a low value compound by the pulp and paper industry, necessitating the development of new applications. Current work proposes an innovative KL utilization strategy that connects partial lignin degradation with lipid production in oleaginous Rhodococcus. Results showed poor bacterial growth when KL was used directly as a substrate. On the other hand, when KL recalcitrance was reduced by oxygen-pretreatment (O-2-KL), Rhodococcus opacus DSM 1069 was capable of utilizing this material and in turn accumulated lipids. The maximum lipid yield was measured to be 0.067 mg ml(-1) at 36 h of growth and these lipids were mainly composed of palmitic (46.9%) and stearic (42.7%) acids.

引用

页码：2784 / 2789

页数：6

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