Oxygen tension regulates NK cells differentiation from hematopoietic stem cells in vitro

被引：7

作者：

Yun, Sohyun ^{[1
,2
]}

Lee, Suk Hyung ^{[1
]}

Yoon, Suk-Ran ^{[1
]}

Myung, Pyung-Keun ^{[2
]}

Choi, Inpyo ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Cell Therapy Res Ctr, Taejon 305806, South Korea

[2] Chungnam Natl Univ, Coll Pharm, Dept Pharm, Taejon, South Korea

来源：

IMMUNOLOGY LETTERS | 2011年 / 137卷 / 1-2期

关键词：

Hypoxia; NK cells; Differentiation; Transcription factors; Hematopoietic stem cells; NATURAL-KILLER-CELL; MYELOID-LEUKEMIC-CELLS; DEVELOPMENTAL PATHWAYS; CYTOTOXICITY RECEPTORS; TRANSCRIPTION FACTOR; MEDIATED CYTOLYSIS; HYPOXIC CONDITIONS; SURFACE-MOLECULE; BONE-MARROW; PROTEIN;

D O I：

10.1016/j.imlet.2011.02.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Natural killer (NK) cells are differentiated from hematopoietic stem cells (HSCs) which are located at the lowest end of an oxygen gradient within the bone marrow (BM). In this report, we investigated whether oxygen tension could affect NK cell differentiation from hematopoietic cells in vitro. we found that hypoxia led to an inhibition of differentiation in NM cells, and increased oxygen supply alleviated this inhibition and restored NM cell differentiation under hypoxic condition. Hypoxia-treated cells demonstrated reduced mRNA expression of transcription factors (TFs) that have important roles in NK cell differentiation, such as EOMES, T-bet, GATA-3 and ETS-1. Moreover, hypoxia-pretreated cells recovered mRNA expression of TFs when the oxygen tension was changed to normoxia. Our findings suggest that oxygen tension modulates in vitro differentiation of NM cells through the regulation of TF expression. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：70 / 77

页数：8

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