Fatty acids selectively inhibit eukaryotic DNA polymerase activities in vitro

被引：192
作者：
Mizushina, Y
Tanaka, N
Yagi, H
Kurosawa, T
Onoue, M
Seto, H
Horie, T
Aoyagi, N
Yamaoka, M
Matsukage, A
Yoshida, S
Sakaguchi, K
机构：
[1] SCI UNIV TOKYO,FAC SCI & TECHNOL,DEPT APPL BIOL SCI,NODA,CHIBA 278,JAPAN
[2] NATL INST BIOSCI & HUMAN TECHNOL,LAB MICROBIAL RESOURCE,TSUKUBA,IBARAKI 305,JAPAN
[3] AICHI CANC CTR,RES INST,CELL BIOL LAB,NAGOYA,AICHI 464,JAPAN
[4] NAGOYA UNIV,SCH MED,DIS MECHANISM & CONTROL RES INST,CANC CELL BIOL LAB,NAGOYA,AICHI 466,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1308卷 / 03期
关键词：
fatty acid; DNA polymerase; enzyme inhibitor;
D O I：
10.1016/0167-4781(96)00121-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The in vitro relationship between eukaryotic DNA polymerases and fatty acids was investigated. Some fatty acids strongly inhibited the activities of DNA polymerase alpha and/or beta in vitro. The kinetics of inhibition by linoleic acid showed that DNA polymerase alpha was non-competitively inhibited with respect to the DNA template and substrate (dTTP), while DNA polymerase beta was inhibited competitively with both DNA and substrate.
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收藏
页码：256 / 262
页数：7
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