Neuroprotective Effect of Prenylated Arylbenzofuran and Flavonoids from Morus alba Fruits on Glutamate-Induced Oxidative Injury in HT22 Hippocampal Cells

被引:56
作者
Seo, Kyeong-Hwa [1 ]
Lee, Dae-Young [2 ]
Jeong, Rak-Hun [1 ]
Lee, Dong-Sung [3 ]
Kim, Young-Eon [4 ]
Hong, Eock-Kee [5 ]
Kim, Youn-Chul [3 ]
Baek, Nam-In [1 ]
机构
[1] Kyung Hee Univ, Grad Sch Biotechnol, Dept Oriental Med Mat & Proc, Yongin 446701, South Korea
[2] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Herbal Crop Utilizat Res Team, Eumseong, South Korea
[3] Wonkwang Univ, Hanbang Body Fluid Res Ctr, Iksan, South Korea
[4] Korea Food Res Inst, Songnam, South Korea
[5] Kangwon Natl Univ, Sch Biotechnol & Bioengn, Chunchon, South Korea
关键词
quantitative analysis; Morus alba L; HT22; artoindonesianin O; morachalcone A; neuroprotection effect; ANTIOXIDANT ACTIVITIES; TOXICITY; MULBERRY; STILBENE; L;
D O I
10.1089/jmf.2014.3196
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A prenylated arylbenzofuran and six flavonoids were isolated from the fruits of Morus alba L. through silica gel, octadecyl silica gel, and Diaion HP-20 column chromatography. Based on the nuclear magnetic resonance, mass spectrometry, and infrared spectroscopic data, the chemical structures of the compounds were determined to be artoindonesianin O (1), isobavachalcone (2), morachalcone A (3), quercetin (4), astragalin (5), isoquercetin (6), and rutin (7). The isolated compounds were evaluated for protection of HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Compounds 1 and 3 exhibited protective effects with EC50 values of 19.7 +/- 1.2 and 35.5 +/- 2.1 mu M, respectively. The major compounds 1-3 and 7 were quantified using liquid chromatography/mass spectrometry analysis and were determined to be 1.88 +/- 2.1, 1.90 +/- 1.8, 0.78 +/- 1.5, and 37.29 +/- 2.2 mg/kg, respectively, in the ethanol extract of M. alba L. fruits.
引用
收藏
页码:403 / 408
页数:6
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