QCM-based immunosensor for rapid detection of Salmonella Typhimurium in food

被引：73

作者：

Fulgione, Andrea ^{[1
,2
]}

Cimafonte, Martina ^{[3
]}

Della Ventura, Bartolomeo ^{[3
]}

Iannaccone, Marco ^{[1
]}

Ambrosino, Concetta ^{[2
]}

Capuano, Federico ^{[4
]}

Proroga, Yolande Therese Rose ^{[4
]}

Velotta, Raffaele ^{[3
]}

Capparelli, Rosanna ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Agr, I-80055 Portici Naples, Italy

[2] Univ Sannio, Dept Sci & Technol, I-82100 Benevento, Italy

[3] Univ Naples Federico II, Dept Phys Ettore Pancini, I-80126 Naples, Italy

[4] Ist Zooprofilatt Sperimentale Mezzogiorno, Dept Food Inspect, I-80055 Portici Naples, Italy

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

关键词：

PHOTOELECTROCHEMICAL IMMUNOASSAY; SENSITIVE DETECTION; FRESH PRODUCE; UNITED-STATES; REAL-TIME; GOLD; MICROBALANCE; ANTIBODIES; BIOSENSORS; FREQUENCY;

D O I：

10.1038/s41598-018-34285-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Salmonella Typhimurium is one of the main causes of outbreaks and sporadic cases of human gastroenteritis. At present, the rapid detection of this pathogen is a major goal of biosensing technology applied to food safety. In fact, ISO standardized culture method takes up to ten days to provide a reliable response. In this paper, we describe a relatively simple protocol for detecting Salmonella Typhimurium in chicken meat based on a Quartz-Crystal Microbalance (QCM), which leads to a limit of detection (LOD) less than of 10 degrees CFU/mL and requires a pre-enrichment step lasting only 2 h at 37 degrees C. The reliability of the proposed immunosensor has been demonstrated through the validation of the experimental results with ISO standardized culture method. The cost-effectiveness of the procedure and the rapidity of the QCM-based biosensor in providing the qualitative response make the analytical method described here suitable for applications in food inspection laboratory and throughout the chain production of food industry.

引用

页数：8

共 48 条

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