Beta-Hemolytic Bacteria Selectively Trigger Liposome Lysis, Enabling Rapid and Accurate Pathogen Detection

被引：15

作者：

Sum, Rongji ^{[1
]}

Swaminathan, Muthukaruppan ^{[1
,2
]}

Rastogi, Sahil Kumar ^{[1
,4
]}

Piloto, Obdulio ^{[3
]}

Cheong, Ian ^{[1
,2
]}

机构：

[1] Temasek Life Sci Lab, Dept Mol Pathogenesis, Singapore 117604, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117558, Singapore

[3] Protean Labs LLC, Medley, FL 33166 USA

[4] Carnegie Mellon Univ, Dept Biomed Engn, Pittsburgh, PA 15213 USA

来源：

ACS SENSORS | 2017年 / 2卷 / 10期

关键词：

hemolysis; sensor; liposome; blood agar; erythrocyte; bacteria; pathogen; image analysis; LISTERIA-MONOCYTOGENES; BLOOD AGAR; PERMEABILITY; DIAGNOSIS; RELEASE; CULTURE;

D O I：

10.1021/acssensors.7b00333

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

For more than a century, blood agar plates have been the only test for beta-hemolysis. Although blood agar cultures are highly predictive for bacterial pathogens, they are too slow to yield actionable information. Here, we show that beta-hemolytic pathogens are able to lyse and release fluorophores encapsulated in sterically stabilized liposomes whereas alpha and gamma-hemolytic bacteria have no effect. By analyzing fluorescence kinetics, beta-hemolytic colonies cultured on agar could be distinguished in real time with 100% accuracy within 6 h. Additionally, end point analysis based on fluorescence intensity and machine-extracted textural features could discriminate between beta-hemolytic and cocultured control colonies with 99% accuracy. In broth cultures, beta-hemolytic bacteria were detectable in under an hour while control bacteria remained negative even the next day. This strategy, called beta-hemolysis triggered-release assay (BETA) has the potential to enable the same-day detection of beta-hemolysis with single-cell sensitivity and high accuracy.

引用

页码：1441 / 1451

页数：11