Mapping of the Rsd contact site on the sigma 70 subunit of Escherichia coli RNA polymerase

被引：32
|
作者：
Jishage, M [1 ]
Dasgupta, D [1 ]
Ishihama, A [1 ]
机构：
[1] Natl Inst Genet, Dept Mol Genet, Shizuoka 4118540, Japan
来源：
JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 09期
关键词：
D O I：
10.1128/JB.183.9.2952-2956.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma (70) subunit. The contact site of Rsd on sigma (70) was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma (70) and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma (70) contact transcription factors.
引用
收藏
页码：2952 / 2956
页数：5
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