RETRACTED: Long Noncoding RNA ZFPM2-AS1 Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression (Retracted article. See vol. 12, pg. 2367, 2020)

被引：8
作者：
Dai, Jun [1 ]
Wei, Rujia [2 ]
Zhang, Peihai [3 ]
Liu, Peishu [1 ]
机构：
[1] Shandong Univ, Dept Gynaecol & Obstet, Qilu Hosp, 107 Wenhua West Rd, Jinan 250012, Shandong, Peoples R China
[2] Liaocheng Univ, Sch Life Sci, Liaocheng 252004, Shandong, Peoples R China
[3] Shandong Univ Qingdao, Qilu Hosp, Dept Gynaecol & Obstet, Qingdao 266035, Shandong, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2020年 / 12卷
关键词：
ZFPM2 antisense RNA 1; cervical cancer therapy; fibroblast growth factor receptor 2; microRNA-511-3p; SQUAMOUS-CELL CARCINOMA; PROGNOSTIC VALUE; UP-REGULATION; MECHANISMS; PROMOTES; PROLIFERATION; PROGRESSION; SUPPRESSES; PREDICTION; GROWTH;
D O I：
10.2147/CMAR.S238373
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Purpose: A long noncoding RNA called ZFPM2 antisense RNA 1 (ZFPM2-AS1) has been verified as a key modulator in multiple human cancer types. Nonetheless, the expression and functions of ZFPM2-AS1 in cervical cancer remain poorly understood. Therefore, our purpose was to characterize the expression pattern, clinical value, and detailed roles of ZFPM2-AS1 in cervical cancer. Methods: Reverse-transcription quantitative PCR was carried out to measure ZFPM2-AS1 expression in cervical cancer. A Cell Counting Kit-8 assay, flow cytometry, Transwell migration and invasion assays, and a tumor xenograft experiment were conducted to determine the influence of ZFPM2-AS1 on cervical cancer cell proliferation, apoptosis, migration, and invasion in vitro and on tumor growth in vivo, respectively. Results: ZFPM2-AS1 was found to be aberrantly upregulated in cervical cancer, and its upregulation was associated with unfavorable values of clinical parameters. A ZFPM2-AS1 knockdown significantly reduced cervical cancer cell proliferation, migration, and invasion and increased apoptosis in vitro. The ZFPM2-AS1 knockdown decelerated tumor growth of cervical cancer cells in vivo. Molecular investigation indicated that ZFPM2-AS1 acts as a molecular sponge of microRNA-511-3p (miR-511-3p) in cervical cancer cells. Fibroblast growth factor receptor 2 (FGFR2) mRNA was validated as a direct target of miR-511-3p in cervical cancer, and its expression was positively modulated by ZFPM2-AS1. The effects of the ZFPM2-AS1 knockdown on malignant characteristics of cervical cancer cells were greatly attenuated by miR-511-3p inhibition. Conclusion: ZFPM2-AS1 promotes cervical cancer progression through upregulation of miR-511-3p-FGFR2 axis output, thereby pointing to possible diagnostics and therapeutics based on the ZFPM2-AS1-miR-511-3p-FGFR2 pathway.
引用
收藏
页码：567 / 580
页数：14
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