Comparative transcriptome analysis revealed gamma-irradiation mediated disruption of floral integrator gene(s) leading to prolonged vegetative phase in Stevia rebaudiana Bertoni

被引：12

作者：

Singh, Gopal ^{[1
,2
]}

Pal, Poonam ^{[1
,2
]}

Masand, Mamta ^{[1
]}

Seth, Romit ^{[1
]}

Kumar, Ashok ^{[3
]}

Singh, Sanatsujat ^{[3
]}

Sharma, Ram Kumar ^{[1
,2
]}

机构：

[1] CSIR Inst Himalayan Bioresource Technol, Biotechnol Div, Palampur 176061, Himachal Prades, India

[2] CSIR Inst Himalayan Bioresource Technol, Acad Sci & Innovat Res AcSIR, Palampur 176061, Himachal Prades, India

[3] CSIR Inst Himalayan Bioresource Technol, Agrotechnol Med Aromat & Commercially Important P, Palampur 176061, Himachal Prades, India

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2020年 / 148卷

关键词：

Floral integrator genes; Gamma-irradiation; Low/no calorie natural sweeteners; Mutation breeding; Stevia; Steviol glycosides; Transcriptome; MADS-BOX GENES; GLYCOSIDE ACCUMULATION; PATHWAY; AUXIN; BIOSYNTHESIS; TRANSITIONS; EXPRESSION; BINDING; SYSTEM; LEAVES;

D O I：

10.1016/j.plaphy.2020.01.004

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Stevia rebaudiana Bert. is getting global attention because of its ability to synthesize commercially important low/no calorie natural sweeteners (LNCSs) steviol glycosides (SGs). Considering, higher accumulation of SGs in vegetative phase followed by decrement during reproductive phase necessitate the understanding of different molecular components of floral transition to develop superior varieties/cultivars with prolonged vegetative phase in Stevie. Current comparative transcriptional analysis of low dose (5 kR) gamma-irradiated mutant genotype (SMG) with prolonged vegetative phase vis-a-vis background genotype (SBG) identified DGEs of major floral transition pathways, and expressed according to their physiological fate irrespective to SMG & SBG. Contrarily, reduced expression of floral integrator genes (FT and LEAFY) in mutant genotype suggests their involvement in prolonged vegetative phase phenotype. Likewise, GO and KEGG enrichment of photosynthesis and carbon assimilation efficiency might be associated with prolonged vegetative phase and higher accumulation of Stevioside content in mutant genotype. Furthermore, deviation of flowering related transcription factors (higher expressions except MIKS-type MADS-box SMG PV compared to SBG F) may possibly be correlated with low expression of floral integrator genes. Findings of current studies will facilitate the genetic manipulations and crop improvement efforts in Stevia through conventional breeding and genome editing approaches for increased SGs biosynthesis.

引用

页码：90 / 102

页数：13