Detection of a novel highly pathogenic H7 influenza virus by duplex real-time reverse transcription polymerase chain reaction

被引：9

作者：

Jia, Weixin ^{[1
,4
]}

Cao, Chenfu ^{[2
]}

Lin, Yanxing ^{[2
]}

Zhong, Liangning ^{[3
]}

Xie, Shumin ^{[1
,4
]}

Wang, Xiao ^{[1
,4
]}

Yin, Sanhong ^{[3
]}

Xu, Zhenna ^{[3
]}

Dai, Yixue ^{[1
,4
]}

Li, Zhixian ^{[1
,4
]}

Niu, Xiao ^{[1
,4
]}

Qi, Wenbao ^{[1
,4
]}

Lu, Tikang ^{[2
]}

Liao, Ming ^{[1
,4
]}

机构：

[1] South China Agr Univ, Coll Vet Med, Natl & Reg Joint Engn Lab Medicament Zoonoses Pre, Guangzhou 510642, Guangdong, Peoples R China

[2] Anim & Plant Inspect & Quarantine Technol Ctr, Shenzhen Entry Exit Inspect & Quarantine Bur, Shenzhen 518054, Peoples R China

[3] Anim Dis Control Ctr Dongguan City, Dongguan 523086, Peoples R China

[4] Minist Agr, Key Lab Zoonoses, Guangzhou 510642, Guangdong, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2017年 / 246卷

关键词：

Highly pathogenic H7 virus; rRT-PCR; H7N9; HEMAGGLUTININ; CLEAVABILITY;

D O I：

10.1016/j.jviromet.2017.03.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

On February 19, 2017, China announced that the mutant H7N9 virus appeared in human cases, which showed molecular characteristic of highly pathogenic virus for poultry. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for distinguish between highly pathogenic H7 virus and low pathogenic H7 virus. The sensitivity, specificity, stability and conformance tests were conducted for this method. The data showed that the new method is sensitive. The minimum detection limit for the RNA of highly pathogenic H7 virus is 0.0052 fg and the minimum detection limit for the RNA of low pathogenic H7 virus is 0.36 fg. The method gave specific results in detecting novel highly pathogenic H7 virus and will play an important role in the rapid identification of novel highly pathogenic H7 virus.

引用

页码：100 / 103

页数：4

共 11 条

[1]

[Anonymous], 2017, HUM INF AV INFL A H7

[2]

Food and Agriculture Organization, 2017, H7N9 SIT UPD 15 FEBR

[3] CLEAVABILITY OF HEMAGGLUTININ FROM AN EXTREMELY VIRULENT-STRAIN OF AVIAN INFLUENZA-VIRUS CONTAINING A UNIQUE CLEAVAGE SITE SEQUENCE [J].