Analysis of nifH-RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2-fixing community depending on depth and agricultural use of soil

被引:33
作者
Calderoli, Priscila A. [1 ]
Collavino, Monica M. [2 ]
Behrends Kraemer, Filipe [3 ,4 ]
Morras, Hector J. M. [4 ]
Mario Aguilar, O. [1 ]
机构
[1] Univ Nacl La Plata, CONICET, IBBM, La Plata, Buenos Aires, Argentina
[2] Univ Nacl Nordeste, CONICET, Fac Ciencias Agr, Inst Bot Nordeste IBONE, Corrientes, Argentina
[3] Univ Buenos Aires, Fac Agron, Catedra Manejo & Conservac Suelos, Buenos Aires, DF, Argentina
[4] Inst Suelos, INTA CIRN, Hurlingham, Provincia De Bu, Argentina
关键词
Cyanobacteria and Geobacter; nifH transcripts; soil diazotrophic community; soil management practices; MICROBIAL COMMUNITIES; NITROGEN-FIXATION; GENE DIVERSITY; BACTERIAL COMMUNITIES; COLORADO PLATEAU; PHYSICAL QUALITY; USE INTENSITY; N-2; FIXATION; LAND-USE; EXPRESSION;
D O I
10.1002/mbo3.502
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N-2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize-soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism's activities, such as free N-2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
引用
收藏
页数:15
相关论文
共 98 条
[91]   Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes [J].
Will, Christiane ;
Thuermer, Andrea ;
Wollherr, Antje ;
Nacke, Heiko ;
Herold, Nadine ;
Schrumpf, Marion ;
Gutknecht, Jessica ;
Wubet, Tesfaye ;
Buscot, Francois ;
Daniel, Rolf .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2010, 76 (20) :6751-6759
[92]   Revisiting N2 fixation in Guerrero Negro intertidal microbial mats with a functional single-cell approach [J].
Woebken, Dagmar ;
Burow, Luke C. ;
Behnam, Faris ;
Mayali, Xavier ;
Schintlmeister, Arno ;
Fleming, Erich D. ;
Prufert-Bebout, Leslie ;
Singer, Steven W. ;
Lopez Cortes, Alejandro ;
Hoehler, Tori M. ;
Pett-Ridge, Jennifer ;
Spormann, Alfred M. ;
Wagner, Michael ;
Weber, Peter K. ;
Bebout, Brad M. .
ISME JOURNAL, 2015, 9 (02) :485-496
[93]   Three distinct clades of cultured heterocystous cyanobacteria constitute the dominant N2-fixing members of biological soil crusts of the Colorado Plateau, USA [J].
Yeager, Chris M. ;
Kornosky, Jennifer L. ;
Morgan, Rachael E. ;
Cain, Elizabeth C. ;
Garcia-Pichel, Ferran ;
Housman, David C. ;
Belnap, Jayne ;
Kuske, Cheryl R. .
FEMS MICROBIOLOGY ECOLOGY, 2007, 60 (01) :85-97
[94]   Diazotrophic community structure and function in two successional stages of biological soil crusts from the Colorado plateau and Chihuahuan desert [J].
Yeager, CM ;
Kornosky, JL ;
Housman, DC ;
Grote, EE ;
Belnap, J ;
Kuske, CR .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (02) :973-983
[95]  
Young JPW., 1992, BIOL NITROGEN FIXATI, P43
[96]   Expression of nifH genes in natural microbial assemblages in Lake George, New York, detected by reverse transcriptase PCR [J].
Zani, S ;
Mellon, MT ;
Collier, JL ;
Zehr, JP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (07) :3119-3124
[97]   Nitrogenase gene diversity and microbial community structure: a cross-system comparison [J].
Zehr, JP ;
Jenkins, BD ;
Short, SM ;
Steward, GF .
ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (07) :539-554
[98]   USE OF DEGENERATE OLIGONUCLEOTIDES FOR AMPLIFICATION OF THE NIFH GENE FROM THE MARINE CYANOBACTERIUM TRICHODESMIUM-THIEBAUTII [J].
ZEHR, JP ;
MCREYNOLDS, LA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (10) :2522-2526