CYP1A mRNA expression in redeye mullets (Liza haematocheila) from Bohai Bay, China

被引：27

作者：

An, Lihui ^{[1
,2
,3
]}

Hu, Jianying ^{[1
]}

Yang, Min ^{[2
]}

Zheng, Binghui ^{[3
]}

Wei, An ^{[1
,2
]}

Shang, Jingjing ^{[3
]}

Zhao, Xingru ^{[3
]}

机构：

[1] Peking Univ, MOE Lab Earth Surface Proc, Coll Urban & Environm Sci, Beijing 100871, Peoples R China

[2] Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

[3] Chinese Res Inst Environm Sci, State Environm Protect Key Lab Estuarine & Coasta, Beijing 100012, Peoples R China

来源：

MARINE POLLUTION BULLETIN | 2011年 / 62卷 / 04期

关键词：

Redeye mullet; CYP1A; Real-time quantitative PCR; Pollutants; POLYBROMINATED DIPHENYL ETHERS; POLYCYCLIC AROMATIC-HYDROCARBONS; MARINE FOOD-WEB; EQUIVALENCY FACTORS TEFS; NORTH CHINA; ORGANOCHLORINE PESTICIDES; POLYCHLORINATED BIPHENYL; ORGANIC POLLUTANTS; EROD ACTIVITY; FISH;

D O I：

10.1016/j.marpolbul.2011.01.019

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Induction of cytochrome P4501A (CYP1A) has been used as a biomarker in fish for monitoring aromatic and organic contaminants. In this study, a partial of CYP1A gene in redeye mullet (Liza haematocheila) was isolated and sequenced, and then a real-time quantitative reverse-transcription polymerase chain reaction assay was developed for quantification of CYP1A mRNA normalized to beta-actin. The developed method was applied to detect CYP1A mRNA expression in redeye mullets collected from Nandaihe (reference site) and Dashentang (impacted site) in Bohai Bay, China. CYP1A mRNA expression values were significantly elevated in redeye mullets from Dashentang compared to a reference site - Nandaihe, which was correlated with the contents of different environmentally relevant pollutants in tissues, particularly with PCBs and PBDEs. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：718 / 725

页数：8

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