Qualitative Evaluation of Platelet Concentrates Prepared with Different Methods

被引:0
|
作者
Varshashree [1 ]
Das, Subhashis [1 ]
Prabhakar, K. [2 ]
机构
[1] Sridevaraj Urs Med Coll, Dept Pathol, Kolar 563101, Karnataka, India
[2] Sridevaraj Urs Med Coll, Dept Med, Kolar, Karnataka, India
关键词
Apheresis; Buffy coat; Whole blood; BUFFY-COAT; TRANSFUSION; APHERESIS;
D O I
10.7860/JCDR/2021/47715.15558
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Introduction: One of the most important responsibility of Blood Bank is to ensure uninterrupted supply of quality blood products, namely Platelet Concentrate (PC), which are in high demand particularly during pandemic. In India, PCs are of three types prepared by different methods like Platelet Rich Plasma-Platelet Concentrate (PRP-PC), Buffy Coat Platelet Concentrates (BC-PC), and Single Donor Platelets units (SDP). Hence, establishing optimum quality standards across all the three types of PC remains a challenge in order to maximum benefit to recipients. Aim: To assess and compare the quality parameters of platelet concentrate prepared by PRP, BC-PC and apheresis methods Materials and Methods: Laboratory based observational study done at rural, tertiary care, post graduate, teaching hospital located at south India from December 2018 to November 2019. A total of 156 PCs were studied with 52 units belonging to PRP method, 52units BC-PC method and the remaining were 52 units of SDP prepared by apheresis method. Quality parameters studied include volume, pH, swirling, White Blood Cell count (WBC count/bag), platelet count/bag and platelet indices. Platelet indices include Mean Platelet Volume (MPV), Platelet distribution width (PDW), and Platelet Large Cell Ratio (PLCR). Arterial Blood Gas (ABG) analysis was also done to assess (pH, pCO(2), pHCO(3-)). The p-value less than 0.05 were considered statistically significant. Statistical Package for the Social Sciences (SPSS) software version 23.0 used for statistical analysis. Results: All the 156 PC units were analysed as per the Director General of Health Services (DGHS) India, criteria. A score of 5 was obtained for 58 out of 156 cases for SDP, 56 out of 156 cases for BC-PC, 42 out of 156 cases for PRP-PC units with respective percentage of 37.1%, 33.3% and 26.9%, respectively. All the PC units were sterile and Red Blood Cell (RBC) contamination was within acceptable limits. Conclusion: In this study, quality parameters of SDP was better than BC-PC and PRP-PC but with improvement and more standardisation of procedures and storage, BC-PC units can give a yield similar to that of SDP units.
引用
收藏
页码:EC29 / EC33
页数:5
相关论文
共 50 条
  • [31] Use of random versus apheresis platelet concentrates
    Andreu, G.
    Vasse, J.
    Sandid, I.
    Tardivel, R.
    Semana, G.
    TRANSFUSION CLINIQUE ET BIOLOGIQUE, 2007, 14 (06) : 514 - 521
  • [32] In vitro evaluation of pathogen inactivated platelet quality: An 8 year experience of routine use in Galicia, Spain
    Castrillo Fernandez, Azucena
    Lanteri, Marion C.
    Arcas Otero, Carina
    Diaz Pereira, Almudena
    Adelantado Perez, Maria
    TRANSFUSION AND APHERESIS SCIENCE, 2019, 58 (01) : 87 - 93
  • [33] What about Platelet Function in Platelet Concentrates?
    Prudent, Michel
    HAMOSTASEOLOGIE, 2020, 40 (04): : 500 - 508
  • [34] Pathogen inactivation of platelet concentrates
    Reesink, H. W.
    Panzer, S.
    McQuilten, Z. K.
    Wood, E. M.
    Marks, C.
    Wendel, S.
    Trigo, F.
    Biagini, S.
    Olyntho, S.
    Devine, D. V.
    Mumford, I.
    Cazenave, J. -P.
    Rasongles, P.
    Garraud, O.
    Richard, P.
    Schooneman, F.
    Vezon, G.
    Al Radwan, R.
    Brand, A.
    Hervig, T.
    Castro, E.
    Lozano, M.
    Navarro, L.
    Puig, L.
    Almazan, C.
    MacLennan, S.
    Cardigan, R.
    Franklin, I. M.
    Prowse, C.
    VOX SANGUINIS, 2010, 99 (01) : 85 - 95
  • [35] Platelet concentrates, from whole blood or collected by apheresis?
    van der Meer, Pieter F.
    TRANSFUSION AND APHERESIS SCIENCE, 2013, 48 (02) : 129 - 131
  • [36] Rational Therapy with Platelet Concentrates
    Hoepner, Gero
    Uzun, Guenalp
    Bakchoul, Tamam
    TRANSFUSIONSMEDIZIN, 2023, 13 (01) : 39 - 51
  • [37] The history of buffy coat platelet concentrates: The Dutch story
    van der Meer, Pieter F.
    Reesink, Hendrik W.
    de Korte, Dirk
    Loos, Johannes A.
    Klei, Thomas R. L.
    VOX SANGUINIS, 2022, 117 (07) : 913 - 919
  • [38] Quality assessment of buffy-coat-derived leucodepleted platelet concentrates in PAS-plasma, prepared by the OrbiSac or TACSI automated system
    Plaza, E. M.
    Cespedes, P.
    Fernandez, H.
    Sanchez-Guiu, M. I.
    Egea, J. M.
    Vicente, V.
    Lozano, M. L.
    Rivera, J.
    VOX SANGUINIS, 2014, 106 (01) : 38 - 44
  • [39] Impedance aggregometric analysis of platelet function of apheresis platelet concentrates as a function of storage time
    Glas, Michael
    Bauer, Janine Viola
    Eichler, Hermann
    Volk, Thomas
    SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2016, 76 (08): : 664 - 670
  • [40] Evaluation of the advantages of platelet concentrates stored at 4°C versus 22°C
    Yang, Jiangcun
    Yin, Wen
    Zhang, Yali
    Sun, Yang
    Ma, Ting
    Gu, Shunli
    Gao, Ying
    Zhang, Xiaole
    Yuan, Jun
    Wang, Wenhua
    TRANSFUSION, 2018, 58 (03) : 736 - 747