Isolation and characterization of two lytic bacteriophages against Staphylococcus aureus from India: newer therapeutic agents against Bovine mastitis

被引：27

作者：

Ganaie, M. Y. ^{[1
]}

Qureshi, S. ^{[1
]}

Kashoo, Z. ^{[1
]}

Wani, S. A. ^{[1
]}

Hussain, M. I. ^{[1
]}

Kumar, R. ^{[2
]}

Maqbool, R. ^{[1
]}

Sikander, P. ^{[1
]}

Banday, M. S. ^{[3
]}

Malla, W. A. ^{[2
]}

Mondal, P. ^{[2
]}

Khan, R. I. N. ^{[2
]}

机构：

[1] SKUAST Kashmir, Div Vet Microbiol & Immunol, FVSC & AH, Srinagar 190006, Jammu & Kashmir, India

[2] ICAR IVRI, Dept Vet Biotechnol, Computat Biol & Genom Facil, Bareilly 243122, Uttar Pradesh, India

[3] Govt Med Coll, Dept Pharmacol, Srinagar 190002, Jammu & Kashmir, India

来源：

VETERINARY RESEARCH COMMUNICATIONS | 2018年 / 42卷 / 04期

关键词：

Staphylococcus aureus; Bacteriophages; Mastitis; Multiplicity of infection; STRUCTURAL PROTEINS; PHAST;

D O I：

10.1007/s11259-018-9736-y

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine mastitis causes severe economic losses to dairy farmers. Staphylococcus aureus, is one of the most important pathogen implicated in etiology of clinical and subclinical mastitis in bovines. In view of increasing antimicrobial resistance alternatives to antibiotic therapy are much needed. The present decade has witnessed a renewed interest in phage based therapeutics and diagnostics. The present study, describes isolation and characterization of two lytic phages SAJK-IND and MSP against Staphylococcus aureus having a potential to be used in therapy against mastitis. SAJK-IND and MSP phages belonged to Myoviridae and Podoviridae families, respectively. TEM imaging of the two phages revealed an iscosahedral head. MSP phage has a short non contractile tail. SAJK-IND and MSP have a burst size of 44 +/- 3 and 25 +/- 5 PFU/infected cell, respectively. SAJK-IND and MSP phages revealed 12 and 16 proteins, respectively on SDS-PAGE analysis. The lytic activity of the phages was specific for Staphylococcus aureus. SAJK-IND revealed 100% lytic activity against several strains of Staphylococcus aureus isolated from mastitis milk samples whereas, MSP had only 40% lytic activity. SAJK-IND phage genome was sequenced, assembled and deposited in Genbank under accession no MG010123.

引用

页码：289 / 295

页数：7

共 26 条

[1]

Abishek MR, 2010, BIOTECHNOL INT, V3, P18

[2] PHASTER: a better, faster version of the PHAST phage search tool [J].

Arndt, David ;

Grant, Jason R. ;

Marcu, Ana ;

Sajed, Tanvir ;

Pon, Allison ;

Liang, Yongjie ;

Wishart, David S. .

NUCLEIC ACIDS RESEARCH, 2016, 44 (W1) :W16-W21

[3]

Boulanger Pascale, 2009, V502, P227, DOI 10.1007/978-1-60327-565-1_13

[4] DETECTION OF STAPHYLOCOCCUS-AUREUS BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF THE NUC GENE [J].

BRAKSTAD, OG ;

AASBAKK, K ;

MAELAND, JA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1654-1660

[5] Bacteriophage-Resistant Staphylococcus aureus Mutant Confers Broad Immunity against Staphylococcal Infection in Mice [J].

Capparelli, Rosanna ;

Nocerino, Nunzia ;

Lanzetta, Rosa ;

Silipo, Alba ;

Amoresano, Angela ;

Giangrande, Chiara ;

Becker, Karsten ;

Blaiotta, Giuseppe ;

Evidente, Antonio ;

Cimmino, Alessio ;

Iannaccone, Marco ;

Parlato, Marianna ;

Medaglia, Chiara ;

Roperto, Sante ;

Roperto, Franco ;

Ramunno, Luigi ;

Iannelli, Domenico .

PLOS ONE, 2010, 5 (07)

[6]

Chandra M, 2011, AFR J MICROBIOL RES, V5, P2044

[7] Isolation and characterization of novel giant Stenotrophomonas maltophilia phage φSMA5 [J].

Chang, HC ;

Chen, CR ;

Lin, JW ;

Shen, GH ;

Chang, KM ;

Tseng, YH ;

Weng, SF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (03) :1387-1393

[8] Structural protein analysis of the polyvalent staphylococcal bacteriophage 812 [J].

Eyer, Ludek ;

Pantucek, Roman ;

Zdrahal, Zbynek ;

Konecna, Hana ;

Kasparek, Petr ;

Ruzickova, Vladislava ;

Hernychova, Lenka ;

Preisler, Jan ;

Doskar, Jiri .

PROTEOMICS, 2007, 7 (01) :64-72

[9] Efficacy of Polyvalent Bacteriophage P-27/HP to Control Multidrug Resistant Staphylococcus aureus Associated with Human Infections [J].

Gupta, Ragini ;

Prasad, Yogendra .

CURRENT MICROBIOLOGY, 2011, 62 (01) :255-260

[10] Taking aim on bacterial pathogens: from phage therapy to enzybiotics [J].

Hermoso, Juan A. ;

Garcia, Jose L. ;

Garcia, Pedro .

CURRENT OPINION IN MICROBIOLOGY, 2007, 10 (05) :461-472

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