Selection of RNA-binding peptides using mRNA-peptide fusions

被引：35

作者：

Barrick, JE ^{[1
]}

Takahashi, TT ^{[1
]}

Balakin, A ^{[1
]}

Roberts, RW ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

METHODS | 2001年 / 23卷 / 03期

关键词：

mRNA-peptide fusions; in vitro selection; lambda-N;

D O I：

10.1006/meth.2000.1139

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have been working to apply in vitro selection to isolate novel RNA-binding peptides. To do this, we use mRNA-protein fusions, peptides covalently attached to their own mRNA. Here, we report selection protocols developed using the arginine-rich domain of bacteriophage lambda-N protein and its binding target, the boxB RNA. Systematic investigation of possible paths for a selection round has allowed us to design a reliable and efficient protocol to enrich RNA-binding peptides from nonfunctional members of a complex mixture. The protocols we have developed should greatly facilitate the isolation of new molecules using the fusion system. (C) 2001 Academic Press.

引用

页码：287 / 293

页数：7

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