Fast purification process optimization using mixed-mode chromatography sorbents in pre-packed mini-columns

被引：42

作者：

Brochier, Virginie Brenac ^{[1
]}

Schapman, Anthony ^{[1
]}

Santambien, Patrick ^{[1
]}

Britsch, Lothar ^{[2
]}

机构：

[1] PALL France, Pall BioSepra, F-95800 Cergy, France

[2] ATOLL GmbH, D-88250 Weingarten, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1177卷 / 02期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

high-throughput screening; MediaScout MiniChrom columns; HEA HyperCel; PPA HyperCel; MEP HyperCel; mixed-mode sorbents;

D O I：

10.1016/j.chroma.2007.08.086

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pre-packed MediaScout (R) MiniChrom columns of 2.5, 5 and 10 mL were investigated for screening three mixed-mode chromatography sorbents (HEA, PPA and MEP HyperCel (TM)). Packing performance was of good quality and the three sorbents displayed higher capacity than traditional HIC sorbents in physiological-like conditions. Each sorbent offered a unique selectivity. Bovine beta-lactoglobulin was partially purified after loading milk whey directly on HEA HyperCel sorbent. The combination of small pre-packed columns and SELDI-MS appeared to be a valuable strategy for high-throughput screening of chromatography sorbents and for enabling rapid process development and optimization. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：226 / 233

页数：8

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